Primer Details for TRINITY_DN38170_c0_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAATGAATGAACTTGTAAATGGAT | CGCGACATTGAACGTTTTGA | Start: 113 |
| Tm | 57.4 | 57.988 | End: 235 |
| Size | 26 | 20 | 123 |
| Primer 2 details |
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| Sequence | AGGAATGAATGAACTTGTAAATGGAT | CGCGACATTGAACGTTTTGAAC | Start: 113 |
| Tm | 57.4 | 59.851 | End: 235 |
| Size | 26 | 22 | 123 |
| Primer 3 details |
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| Sequence | AGGAATGAATGAACTTGTAAATGGAT | CGCGACATTGAACGTTTTGAA | Start: 113 |
| Tm | 57.4 | 58.632 | End: 235 |
| Size | 26 | 21 | 123 |
| Primer 4 details |
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| Sequence | AGGAATGAATGAACTTGTAAATGGATA | CGCGACATTGAACGTTTTGA | Start: 113 |
| Tm | 57.301 | 57.988 | End: 235 |
| Size | 27 | 20 | 123 |
| Primer 5 details |
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| Sequence | TAGGAATGAATGAACTTGTAAATGGAT | CGCGACATTGAACGTTTTGA | Start: 112 |
| Tm | 57.301 | 57.988 | End: 235 |
| Size | 27 | 20 | 124 |
