Primer Details for TRINITY_DN3648_c0_g1_i1 |
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| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGTGAGAAGTGTTCAGGGC | ACATCTCGAATTACTTAAGGAGAGTGA | Start: 83 |
| Tm | 59.966 | 59.66 | End: 274 |
| Size | 20 | 27 | 192 |
| Primer 2 details |
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| Sequence | CGTGAGAAGTGTTCAGGGCT | ACATCTCGAATTACTTAAGGAGAGTGA | Start: 84 |
| Tm | 59.966 | 59.66 | End: 274 |
| Size | 20 | 27 | 191 |
| Primer 3 details |
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| Sequence | TCGTGAGAAGTGTTCAGGGC | ACATCTCGAATTACTTAAGGAGAGTG | Start: 83 |
| Tm | 59.966 | 58.515 | End: 274 |
| Size | 20 | 26 | 192 |
| Primer 4 details |
|---|
| Sequence | CGTGAGAAGTGTTCAGGGCT | ACATCTCGAATTACTTAAGGAGAGTG | Start: 84 |
| Tm | 59.966 | 58.515 | End: 274 |
| Size | 20 | 26 | 191 |
| Primer 5 details |
|---|
| Sequence | TCGTGAGAAGTGTTCAGGGC | ACATCTCGAATTACTTAAGGAGAGT | Start: 83 |
| Tm | 59.966 | 57.352 | End: 274 |
| Size | 20 | 25 | 192 |
