Primer Details for TRINITY_DN36191_c0_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGATTTTGACAAACTATTTACACA | TCCGAGGGTTTTGAAATTAGCA | Start: 52 |
| Tm | 57.528 | 58.243 | End: 228 |
| Size | 26 | 22 | 177 |
| Primer 2 details |
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| Sequence | AGGGATTTTGACAAACTATTTACACA | TCCGAGGGTTTTGAAATTAGCAA | Start: 52 |
| Tm | 57.528 | 58.854 | End: 228 |
| Size | 26 | 23 | 177 |
| Primer 3 details |
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| Sequence | AAGGGATTTTGACAAACTATTTACACA | TCCGAGGGTTTTGAAATTAGCA | Start: 51 |
| Tm | 58.081 | 58.243 | End: 228 |
| Size | 27 | 22 | 178 |
| Primer 4 details |
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| Sequence | AGGGATTTTGACAAACTATTTACACAT | TCCGAGGGTTTTGAAATTAGCA | Start: 52 |
| Tm | 57.911 | 58.243 | End: 228 |
| Size | 27 | 22 | 177 |
| Primer 5 details |
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| Sequence | AGGGATTTTGACAAACTATTTACACA | CCGAGGGTTTTGAAATTAGCAA | Start: 52 |
| Tm | 57.528 | 57.487 | End: 227 |
| Size | 26 | 22 | 176 |
