ssr_primer
Primer Details for TRINITY_DN3571_c0_g1_i1 |
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| (A)10gtccgactcccccgatggaaaataaaaaggagagaaaaaacaaaaaacgaagagaaaaaaacttccatcgc(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAGTCCGATTCCCCTTGGA | ATTTTCCATCGGGGGAGTCG | Start: 12 |
| Tm | 59.957 | 59.82 | End: 140 |
| Size | 20 | 20 | 129 |
| Primer 2 details |
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| Sequence | CGACTCCCCCGATGGAAAAT | TTTTGGCCGACGTTTCATCG | Start: 121 |
| Tm | 59.82 | 59.764 | End: 340 |
| Size | 20 | 20 | 220 |
| Primer 3 details |
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| Sequence | CGACTCCCCCGATGGAAAAT | CGGGGATGGAGGACCTTTTT | Start: 121 |
| Tm | 59.82 | 59.668 | End: 314 |
| Size | 20 | 20 | 194 |
| Primer 4 details |
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| Sequence | AGAGTCCGATTCCCCTTGGA | ATCGGGGGAGTCGGACTTTT | Start: 12 |
| Tm | 59.957 | 60.908 | End: 133 |
| Size | 20 | 20 | 122 |
| Primer 5 details |
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| Sequence | AAGAGTCCGATTCCCCTTGG | ATTTTCCATCGGGGGAGTCG | Start: 11 |
| Tm | 59.088 | 59.82 | End: 140 |
| Size | 20 | 20 | 130 |
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