ssr_primer
Primer Details for TRINITY_DN33574_c0_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGTTATATGGGGCGCCAGC | GGATGCGGGTCCCCTTTTT | Start: 132 |
| Tm | 60.822 | 60.305 | End: 265 |
| Size | 20 | 19 | 134 |
| Primer 2 details |
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| Sequence | TGTTATATGGGGCGCCAGC | GGATGCGGGTCCCCTTTTT | Start: 133 |
| Tm | 60.228 | 60.305 | End: 265 |
| Size | 19 | 19 | 133 |
| Primer 3 details |
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| Sequence | GCTTATTGTTATATGGGGCGCC | ATTTGCCACGTGGAACAACC | Start: 127 |
| Tm | 59.836 | 59.612 | End: 227 |
| Size | 22 | 20 | 101 |
| Primer 4 details |
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| Sequence | CGGTGTCCGGACAGAAAAAT | GGATGCGGGTCCCCTTTTT | Start: 163 |
| Tm | 58.481 | 60.305 | End: 265 |
| Size | 20 | 19 | 103 |
| Primer 5 details |
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| Sequence | TTGTTATATGGGGCGCCAGC | GGATGCGGGTCCCCTTTT | Start: 132 |
| Tm | 60.822 | 59.641 | End: 265 |
| Size | 20 | 18 | 134 |
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