Primer Details for TRINITY_DN31372_c0_g1_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGGGTGCTGTTGGTACGAG | ACCTTTCCAAAAAGCATTTGAAAGT | Start: 184 |
| Tm | 59.965 | 58.82 | End: 305 |
| Size | 20 | 25 | 122 |
| Primer 2 details |
|---|
| Sequence | TGCTGTTGGTACGAGGCAAA | ACCTTTCCAAAAAGCATTTGAAAGT | Start: 189 |
| Tm | 60.179 | 58.82 | End: 305 |
| Size | 20 | 25 | 117 |
| Primer 3 details |
|---|
| Sequence | CAAGGGTGCTGTTGGTACGA | ACCTTTCCAAAAAGCATTTGAAAGT | Start: 183 |
| Tm | 60.25 | 58.82 | End: 305 |
| Size | 20 | 25 | 123 |
| Primer 4 details |
|---|
| Sequence | TCAAGGGTGCTGTTGGTACG | ACCTTTCCAAAAAGCATTTGAAAGT | Start: 182 |
| Tm | 60.25 | 58.82 | End: 305 |
| Size | 20 | 25 | 124 |
| Primer 5 details |
|---|
| Sequence | AAGGGTGCTGTTGGTACGAG | AACCTTTCCAAAAAGCATTTGAAAGT | Start: 184 |
| Tm | 59.965 | 59.341 | End: 306 |
| Size | 20 | 26 | 123 |
(TTA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGGGTGCTGTTGGTACGAG | ACCTTTCCAAAAAGCATTTGAAAGT | Start: 184 |
| Tm | 59.965 | 58.82 | End: 305 |
| Size | 20 | 25 | 122 |
| Primer 2 details |
|---|
| Sequence | TGCTGTTGGTACGAGGCAAA | ACCTTTCCAAAAAGCATTTGAAAGT | Start: 189 |
| Tm | 60.179 | 58.82 | End: 305 |
| Size | 20 | 25 | 117 |
| Primer 3 details |
|---|
| Sequence | CAAGGGTGCTGTTGGTACGA | ACCTTTCCAAAAAGCATTTGAAAGT | Start: 183 |
| Tm | 60.25 | 58.82 | End: 305 |
| Size | 20 | 25 | 123 |
| Primer 4 details |
|---|
| Sequence | TCAAGGGTGCTGTTGGTACG | ACCTTTCCAAAAAGCATTTGAAAGT | Start: 182 |
| Tm | 60.25 | 58.82 | End: 305 |
| Size | 20 | 25 | 124 |
| Primer 5 details |
|---|
| Sequence | AAGGGTGCTGTTGGTACGAG | AACCTTTCCAAAAAGCATTTGAAAGT | Start: 184 |
| Tm | 59.965 | 59.341 | End: 306 |
| Size | 20 | 26 | 123 |
