Primer Details for TRINITY_DN30500_c0_g1_i1 |
|---|
| (A)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCGGTTTTTGGTGTAAATAGATGT | TGCAGGTTTTGGGTTGGTCA | Start: 96 |
| Tm | 59.524 | 60.325 | End: 210 |
| Size | 25 | 20 | 115 |
| Primer 2 details |
|---|
| Sequence | GCGGTTTTTGGTGTAAATAGATGT | TGCAGGTTTTGGGTTGGTCA | Start: 97 |
| Tm | 58.294 | 60.325 | End: 210 |
| Size | 24 | 20 | 114 |
| Primer 3 details |
|---|
| Sequence | AGCGGTTTTTGGTGTAAATAGATG | TGCAGGTTTTGGGTTGGTCA | Start: 96 |
| Tm | 58.05 | 60.325 | End: 210 |
| Size | 24 | 20 | 115 |
| Primer 4 details |
|---|
| Sequence | AGCGGTTTTTGGTGTAAATAGATGTT | TGCAGGTTTTGGGTTGGTCA | Start: 96 |
| Tm | 60.016 | 60.325 | End: 210 |
| Size | 26 | 20 | 115 |
| Primer 5 details |
|---|
| Sequence | GCGGTTTTTGGTGTAAATAGATGTT | TGCAGGTTTTGGGTTGGTCA | Start: 97 |
| Tm | 58.846 | 60.325 | End: 210 |
| Size | 25 | 20 | 114 |
