Primer Details for TRINITY_DN30270_c0_g1_i1 |
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| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCAGAGAGTTTTATTGGTTTTCGT | AGATGAAACCAAGGGAAGAGGA | Start: 29 |
| Tm | 57.438 | 58.74 | End: 135 |
| Size | 25 | 22 | 107 |
| Primer 2 details |
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| Sequence | TCAGAGAGTTTTATTGGTTTTCGTAGT | AGATGAAACCAAGGGAAGAGGA | Start: 30 |
| Tm | 59.07 | 58.74 | End: 135 |
| Size | 27 | 22 | 106 |
| Primer 3 details |
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| Sequence | TTCAGAGAGTTTTATTGGTTTTCGT | AAGATGAAACCAAGGGAAGAGGA | Start: 29 |
| Tm | 57.438 | 59.342 | End: 136 |
| Size | 25 | 23 | 108 |
| Primer 4 details |
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| Sequence | GGTTTTCGTAGTTTCATCTTGAACCT | ACAAAGATCAAGATGAAACCAAGGG | Start: 45 |
| Tm | 60.017 | 59.755 | End: 145 |
| Size | 26 | 25 | 101 |
| Primer 5 details |
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| Sequence | TTCAGAGAGTTTTATTGGTTTTCGT | GATGAAACCAAGGGAAGAGGA | Start: 29 |
| Tm | 57.438 | 57.279 | End: 134 |
| Size | 25 | 21 | 106 |
