Primer Details for TRINITY_DN25129_c0_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCACTGCTTTTTCCGAGCG | TTTTCATCCTAATTTCTAGCATACGAA | Start: 129 |
| Tm | 60.111 | 57.392 | End: 237 |
| Size | 20 | 27 | 109 |
| Primer 2 details |
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| Sequence | GTCACTGCTTTTTCCGAGCG | TTTTTCATCCTAATTTCTAGCATACGA | Start: 129 |
| Tm | 60.111 | 57.392 | End: 238 |
| Size | 20 | 27 | 110 |
| Primer 3 details |
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| Sequence | TCACTGCTTTTTCCGAGCGA | TTTTCATCCTAATTTCTAGCATACGAA | Start: 130 |
| Tm | 60.25 | 57.392 | End: 237 |
| Size | 20 | 27 | 108 |
| Primer 4 details |
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| Sequence | TCACTGCTTTTTCCGAGCGA | TTTTTCATCCTAATTTCTAGCATACGA | Start: 130 |
| Tm | 60.25 | 57.392 | End: 238 |
| Size | 20 | 27 | 109 |
| Primer 5 details |
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| Sequence | GTCACTGCTTTTTCCGAGCG | TCATCCTAATTTCTAGCATACGAATTT | Start: 129 |
| Tm | 60.111 | 57.221 | End: 234 |
| Size | 20 | 27 | 106 |
