ssr_primer
Primer Details for TRINITY_DN22872_c0_g1_i1 |
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| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGTCGACTTTATGGGCCA | TTTCCGTCCAGGCCTCAAAC | Start: 224 |
| Tm | 59.312 | 60.536 | End: 355 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
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| Sequence | TGTGTCGACTTTATGGGCCA | GTTTCCGTCCAGGCCTCAAA | Start: 224 |
| Tm | 59.312 | 60.536 | End: 356 |
| Size | 20 | 20 | 133 |
| Primer 3 details |
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| Sequence | TGTGTCGACTTTATGGGCCA | TAGTTTCCGTCCAGGCCTCA | Start: 224 |
| Tm | 59.312 | 60.543 | End: 358 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
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| Sequence | TGGAACTTGGCCCAGAAACC | CACTCGGCCCAATCCATGAG | Start: 286 |
| Tm | 60.469 | 60.817 | End: 385 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
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| Sequence | TGTGTCGACTTTATGGGCCA | TTAGTTTCCGTCCAGGCCTC | Start: 224 |
| Tm | 59.312 | 59.388 | End: 359 |
| Size | 20 | 20 | 136 |
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