Primer Details for TRINITY_DN22054_c0_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAGAAATATAGTTATATGCTGGCGC | ATGTCACAATTATACGGGATGCG | Start: 113 |
| Tm | 60.238 | 59.254 | End: 212 |
| Size | 26 | 23 | 100 |
| Primer 2 details |
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| Sequence | TCCAGAAATATAGTTATATGCTGGCG | TGTCACAATTATACGGGATGCG | Start: 112 |
| Tm | 58.958 | 58.872 | End: 211 |
| Size | 26 | 22 | 100 |
| Primer 3 details |
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| Sequence | AGCCCTCTATAATTGTTTACTCATCCA | TGTCACAATTATACGGGATGCG | Start: 89 |
| Tm | 59.928 | 58.872 | End: 211 |
| Size | 27 | 22 | 123 |
| Primer 4 details |
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| Sequence | TCCAGAAATATAGTTATATGCTGGCG | TGTCACAATTATACGGGATGCGA | Start: 112 |
| Tm | 58.958 | 60.181 | End: 211 |
| Size | 26 | 23 | 100 |
| Primer 5 details |
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| Sequence | AGCCCTCTATAATTGTTTACTCATCCA | TGTCACAATTATACGGGATGCGA | Start: 89 |
| Tm | 59.928 | 60.181 | End: 211 |
| Size | 27 | 23 | 123 |
