Primer Details for TRINITY_DN21978_c0_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGACTTTAGGAGGTGGACCA | AGATGATAAATTATGCACCAACATCA | Start: 67 |
| Tm | 60.203 | 57.532 | End: 263 |
| Size | 21 | 26 | 197 |
| Primer 2 details |
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| Sequence | CGACTTTAGGAGGTGGACCA | AGATGATAAATTATGCACCAACATCA | Start: 68 |
| Tm | 58.737 | 57.532 | End: 263 |
| Size | 20 | 26 | 196 |
| Primer 3 details |
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| Sequence | CTCGACTTTAGGAGGTGGACC | AGATGATAAATTATGCACCAACATCA | Start: 66 |
| Tm | 59.523 | 57.532 | End: 263 |
| Size | 21 | 26 | 198 |
| Primer 4 details |
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| Sequence | TCGACTTTAGGAGGTGGACC | AGATGATAAATTATGCACCAACATCA | Start: 67 |
| Tm | 58.446 | 57.532 | End: 263 |
| Size | 20 | 26 | 197 |
| Primer 5 details |
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| Sequence | TCGACTTTAGGAGGTGGACCA | AGATGATAAATTATGCACCAACATCAA | Start: 67 |
| Tm | 60.203 | 58.084 | End: 263 |
| Size | 21 | 27 | 197 |
