Primer Details for TRINITY_DN20251_c0_g1_i1 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGGCAATGAACCATTTAGAACT | ACACTTGTCGTTGTCATTGAGA | Start: 32 |
| Tm | 58.92 | 58.208 | End: 138 |
| Size | 24 | 22 | 107 |
| Primer 2 details | |||
| Sequence | ACAGGCAATGAACCATTTAGAAC | ACACTTGTCGTTGTCATTGAGA | Start: 32 |
| Tm | 57.609 | 58.208 | End: 138 |
| Size | 23 | 22 | 107 |
| Primer 3 details | |||
| Sequence | AACAGGCAATGAACCATTTAGAACT | ACACTTGTCGTTGTCATTGAGA | Start: 31 |
| Tm | 59.46 | 58.208 | End: 138 |
| Size | 25 | 22 | 108 |
| Primer 4 details | |||
| Sequence | CAGGCAATGAACCATTTAGAACT | ACACTTGTCGTTGTCATTGAGA | Start: 33 |
| Tm | 57.35 | 58.208 | End: 138 |
| Size | 23 | 22 | 106 |
| Primer 5 details | |||
| Sequence | ACAGGCAATGAACCATTTAGAACT | ACACTTGTCGTTGTCATTGAGAT | Start: 32 |
| Tm | 58.92 | 58.619 | End: 138 |
| Size | 24 | 23 | 107 |
