Primer Details for TRINITY_DN20150_c0_g1_i1 |
|---|
| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCATTTGTGTACTTCTAAAAGCGATGA | TCCACTGAGGATTGCTTCTTCC | Start: 20 |
| Tm | 59.613 | 60.027 | End: 179 |
| Size | 27 | 22 | 160 |
| Primer 2 details |
|---|
| Sequence | TTCATTTGTGTACTTCTAAAAGCGA | TCCACTGAGGATTGCTTCTTCC | Start: 19 |
| Tm | 57.515 | 60.027 | End: 179 |
| Size | 25 | 22 | 161 |
| Primer 3 details |
|---|
| Sequence | CATTTGTGTACTTCTAAAAGCGATGA | TCCACTGAGGATTGCTTCTTCC | Start: 21 |
| Tm | 58.482 | 60.027 | End: 179 |
| Size | 26 | 22 | 159 |
| Primer 4 details |
|---|
| Sequence | TCATTTGTGTACTTCTAAAAGCGATG | TCCACTGAGGATTGCTTCTTCC | Start: 20 |
| Tm | 58.482 | 60.027 | End: 179 |
| Size | 26 | 22 | 160 |
| Primer 5 details |
|---|
| Sequence | TGGTCTTCATTTTCATTTGTGTACT | TCCACTGAGGATTGCTTCTTCC | Start: 8 |
| Tm | 57.419 | 60.027 | End: 179 |
| Size | 25 | 22 | 172 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCATTTGTGTACTTCTAAAAGCGATGA | TCCACTGAGGATTGCTTCTTCC | Start: 20 |
| Tm | 59.613 | 60.027 | End: 179 |
| Size | 27 | 22 | 160 |
| Primer 2 details |
|---|
| Sequence | TTCATTTGTGTACTTCTAAAAGCGA | TCCACTGAGGATTGCTTCTTCC | Start: 19 |
| Tm | 57.515 | 60.027 | End: 179 |
| Size | 25 | 22 | 161 |
| Primer 3 details |
|---|
| Sequence | CATTTGTGTACTTCTAAAAGCGATGA | TCCACTGAGGATTGCTTCTTCC | Start: 21 |
| Tm | 58.482 | 60.027 | End: 179 |
| Size | 26 | 22 | 159 |
| Primer 4 details |
|---|
| Sequence | TCATTTGTGTACTTCTAAAAGCGATG | TCCACTGAGGATTGCTTCTTCC | Start: 20 |
| Tm | 58.482 | 60.027 | End: 179 |
| Size | 26 | 22 | 160 |
| Primer 5 details |
|---|
| Sequence | TGGTCTTCATTTTCATTTGTGTACT | TCCACTGAGGATTGCTTCTTCC | Start: 8 |
| Tm | 57.419 | 60.027 | End: 179 |
| Size | 25 | 22 | 172 |
