Primer Details for TRINITY_DN18087_c0_g1_i1 |
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| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAGGAAAAAGAGAGAATGAAAATGTG | TGGAGACCACACAAAATGCCT | Start: 0 |
| Tm | 59.188 | 60.134 | End: 106 |
| Size | 27 | 21 | 107 |
| Primer 2 details |
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| Sequence | AGGCATTTTGTGTGGTCTCCA | AAACCAACAATCAATAGTAGTGGTT | Start: 86 |
| Tm | 60.134 | 57.167 | End: 186 |
| Size | 21 | 25 | 101 |
| Primer 3 details |
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| Sequence | AGGCATTTTGTGTGGTCTCCA | AAAACCAACAATCAATAGTAGTGGT | Start: 86 |
| Tm | 60.134 | 57.167 | End: 187 |
| Size | 21 | 25 | 102 |
| Primer 4 details |
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| Sequence | GGAGGAAAAAGAGAGAATGAAAATGT | TGGAGACCACACAAAATGCCT | Start: 0 |
| Tm | 58.109 | 60.134 | End: 106 |
| Size | 26 | 21 | 107 |
| Primer 5 details |
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| Sequence | GGAGGAAAAAGAGAGAATGAAAATGTG | GGAGACCACACAAAATGCCTG | Start: 0 |
| Tm | 59.188 | 59.729 | End: 105 |
| Size | 27 | 21 | 106 |
