Primer Details for TRINITY_DN1758_c0_g1_i1 | |||
|---|---|---|---|
| (T)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCATTGGAGAAGATCAGAAGAGT | CCTCTCCCCTCCGGTGATTA | Start: 7 |
| Tm | 59.218 | 60.106 | End: 209 |
| Size | 24 | 20 | 203 |
| Primer 2 details | |||
| Sequence | TTTCATGCACATGCGTATTACA | CCTCTCCCCTCCGGTGATTA | Start: 98 |
| Tm | 57.046 | 60.106 | End: 209 |
| Size | 22 | 20 | 112 |
| Primer 3 details | |||
| Sequence | AGACCATTGGAGAAGATCAGAAGA | CCTCTCCCCTCCGGTGATTA | Start: 5 |
| Tm | 58.971 | 60.106 | End: 209 |
| Size | 24 | 20 | 205 |
| Primer 4 details | |||
| Sequence | GACCATTGGAGAAGATCAGAAGAGT | CCTCTCCCCTCCGGTGATTA | Start: 6 |
| Tm | 60.105 | 60.106 | End: 209 |
| Size | 25 | 20 | 204 |
| Primer 5 details | |||
| Sequence | CCATTGGAGAAGATCAGAAGAGTG | CCTCTCCCCTCCGGTGATTA | Start: 8 |
| Tm | 58.882 | 60.106 | End: 209 |
| Size | 24 | 20 | 202 |
