Primer Details for TRINITY_DN15417_c0_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTCATTTTGTTTGGTTTTAGGAA | TCACTAAACTCTCATTGGCTCTCT | Start: 33 |
| Tm | 57.575 | 59.225 | End: 160 |
| Size | 25 | 24 | 128 |
| Primer 2 details |
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| Sequence | TTCCTCATTTTGTTTGGTTTTAGGA | TCACTAAACTCTCATTGGCTCTCT | Start: 32 |
| Tm | 57.575 | 59.225 | End: 160 |
| Size | 25 | 24 | 129 |
| Primer 3 details |
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| Sequence | TCCTCATTTTGTTTGGTTTTAGGAA | ACATCACTAAACTCTCATTGGCTCT | Start: 33 |
| Tm | 57.575 | 60.046 | End: 163 |
| Size | 25 | 25 | 131 |
| Primer 4 details |
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| Sequence | TTCCTCATTTTGTTTGGTTTTAGGA | ACATCACTAAACTCTCATTGGCTCT | Start: 32 |
| Tm | 57.575 | 60.046 | End: 163 |
| Size | 25 | 25 | 132 |
| Primer 5 details |
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| Sequence | TCCTCATTTTGTTTGGTTTTAGGAA | TCACTAAACTCTCATTGGCTCTC | Start: 33 |
| Tm | 57.575 | 57.922 | End: 160 |
| Size | 25 | 23 | 128 |
