ssr_primer
Primer Details for TRINITY_DN14425_c4_g7_i1 |
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| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCCGGACATCTCTACCTCC | GGCCAACCTAGCAAAGCCTA | Start: 270 |
| Tm | 60.106 | 60.034 | End: 515 |
| Size | 20 | 20 | 246 |
| Primer 2 details |
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| Sequence | GAACCTGGGGGATCGAAAGG | GGCCAACCTAGCAAAGCCTA | Start: 247 |
| Tm | 60.107 | 60.034 | End: 515 |
| Size | 20 | 20 | 269 |
| Primer 3 details |
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| Sequence | GGAACCTGGGGGATCGAAAG | GGCCAACCTAGCAAAGCCTA | Start: 246 |
| Tm | 60.107 | 60.034 | End: 515 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
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| Sequence | TAGGCTTTGCTAGGTTGGCC | AACATTGCAGTATGGCGTGC | Start: 496 |
| Tm | 60.034 | 59.829 | End: 693 |
| Size | 20 | 20 | 198 |
| Primer 5 details |
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| Sequence | TTGAAGGACAAGTGGGGGTG | GGCCAACCTAGCAAAGCCTA | Start: 414 |
| Tm | 59.815 | 60.034 | End: 515 |
| Size | 20 | 20 | 102 |
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