Primer Details for TRINITY_DN14281_c3_g7_i1 | |||
|---|---|---|---|
| (AT)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAGGTTTGAGTCGTTTGGC | TGCGAAATACATCTTAGCACACA | Start: 93 |
| Tm | 59.408 | 58.75 | End: 192 |
| Size | 20 | 23 | 100 |
| Primer 2 details | |||
| Sequence | AGGTTTGAGTCGTTTGGCCT | AGTTTCGTTTTTGTCATTTTCGCA | Start: 95 |
| Tm | 59.817 | 59.14 | End: 295 |
| Size | 20 | 24 | 201 |
| Primer 3 details | |||
| Sequence | AGGTTTGAGTCGTTTGGCCT | GAGTTTCGTTTTTGTCATTTTCGCA | Start: 95 |
| Tm | 59.817 | 59.996 | End: 296 |
| Size | 20 | 25 | 202 |
| Primer 4 details | |||
| Sequence | AGGTTTGAGTCGTTTGGCCT | TGAGTTTCGTTTTTGTCATTTTCGC | Start: 95 |
| Tm | 59.817 | 59.996 | End: 297 |
| Size | 20 | 25 | 203 |
| Primer 5 details | |||
| Sequence | GGAGGTTTGAGTCGTTTGGC | TGCGAAATACATCTTAGCACACAA | Start: 93 |
| Tm | 59.408 | 59.307 | End: 192 |
| Size | 20 | 24 | 100 |
