ssr_primer
Primer Details for TRINITY_DN14270_c1_g2_i4 |
|---|
| (A)30: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTGCCGGCCATACAATTCG | CGCGTCGTCTTGTTTTGGTT | Start: 227 |
| Tm | 59.971 | 59.973 | End: 488 |
| Size | 20 | 20 | 262 |
| Primer 2 details |
|---|
| Sequence | CTTGCCGGCCATACAATTCG | TTATTAGGACCGCCGGATGC | Start: 227 |
| Tm | 59.971 | 59.966 | End: 432 |
| Size | 20 | 20 | 206 |
| Primer 3 details |
|---|
| Sequence | AAAAGCGACTTCTAGGGGGC | AATCTCCCCCGCAACAATGT | Start: 1967 |
| Tm | 60.035 | 59.96 | End: 2076 |
| Size | 20 | 20 | 110 |
| Primer 4 details |
|---|
| Sequence | AACCAAAACAAGACGACGCG | GTGAAGGTGGGACCCAACAA | Start: 469 |
| Tm | 59.973 | 60.106 | End: 589 |
| Size | 20 | 20 | 121 |
| Primer 5 details |
|---|
| Sequence | GCATCCGGCGGTCCTAATAA | GTGAAGGTGGGACCCAACAA | Start: 413 |
| Tm | 59.966 | 60.106 | End: 589 |
| Size | 20 | 20 | 177 |
(TC)8: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTGCCGGCCATACAATTCG | CGCGTCGTCTTGTTTTGGTT | Start: 227 |
| Tm | 59.971 | 59.973 | End: 488 |
| Size | 20 | 20 | 262 |
| Primer 2 details |
|---|
| Sequence | CTTGCCGGCCATACAATTCG | TTATTAGGACCGCCGGATGC | Start: 227 |
| Tm | 59.971 | 59.966 | End: 432 |
| Size | 20 | 20 | 206 |
| Primer 3 details |
|---|
| Sequence | AAAAGCGACTTCTAGGGGGC | AATCTCCCCCGCAACAATGT | Start: 1967 |
| Tm | 60.035 | 59.96 | End: 2076 |
| Size | 20 | 20 | 110 |
| Primer 4 details |
|---|
| Sequence | AACCAAAACAAGACGACGCG | GTGAAGGTGGGACCCAACAA | Start: 469 |
| Tm | 59.973 | 60.106 | End: 589 |
| Size | 20 | 20 | 121 |
| Primer 5 details |
|---|
| Sequence | GCATCCGGCGGTCCTAATAA | GTGAAGGTGGGACCCAACAA | Start: 413 |
| Tm | 59.966 | 60.106 | End: 589 |
| Size | 20 | 20 | 177 |
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