ssr_primer
Primer Details for TRINITY_DN14212_c1_g1_i4 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGTCCTCTTGCCAATGCTC | TTTTTGACACCCCACGACCA | Start: 3244 |
| Tm | 60.035 | 60.034 | End: 3360 |
| Size | 20 | 20 | 117 |
| Primer 2 details |
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| Sequence | TTGTCGGCGGTGACCAAATA | TTTTTGACACCCCACGACCA | Start: 3129 |
| Tm | 59.965 | 60.034 | End: 3360 |
| Size | 20 | 20 | 232 |
| Primer 3 details |
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| Sequence | TTGTCGGCGGTGACCAAATA | GAGCATTGGCAAGAGGACCT | Start: 3129 |
| Tm | 59.965 | 60.035 | End: 3263 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
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| Sequence | TGATTGTCAGAGTCGGCCAC | GAGCATTGGCAAGAGGACCT | Start: 3064 |
| Tm | 60.037 | 60.035 | End: 3263 |
| Size | 20 | 20 | 200 |
| Primer 5 details |
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| Sequence | TGTCAGAGTCGGCCACTAGA | GAGCATTGGCAAGAGGACCT | Start: 3068 |
| Tm | 59.962 | 60.035 | End: 3263 |
| Size | 20 | 20 | 196 |
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