ssr_primer
Primer Details for TRINITY_DN14097_c2_g1_i3 |
|---|
| (AG)9: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCCTAGAACTCCACCGGA | ACGACCACGAGCATGAACAT | Start: 1631 |
| Tm | 60.033 | 60.037 | End: 1883 |
| Size | 20 | 20 | 253 |
| Primer 2 details |
|---|
| Sequence | TCCTCCTAGAACTCCACCGG | ACGACCACGAGCATGAACAT | Start: 1630 |
| Tm | 60.033 | 60.037 | End: 1883 |
| Size | 20 | 20 | 254 |
| Primer 3 details |
|---|
| Sequence | TCCATGCGATTGTTGCTCCT | ACGACCACGAGCATGAACAT | Start: 1614 |
| Tm | 60.035 | 60.037 | End: 1883 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
|---|
| Sequence | GTTTTCCTCCGCCGAAAACC | ACGACCACGAGCATGAACAT | Start: 1686 |
| Tm | 60.04 | 60.037 | End: 1883 |
| Size | 20 | 20 | 198 |
| Primer 5 details |
|---|
| Sequence | AATCTCTACCACTGGGCCCT | CCGGTGGAGTTCTAGGAGGA | Start: 1450 |
| Tm | 59.955 | 60.033 | End: 1649 |
| Size | 20 | 20 | 200 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCCTAGAACTCCACCGGA | ACGACCACGAGCATGAACAT | Start: 1631 |
| Tm | 60.033 | 60.037 | End: 1883 |
| Size | 20 | 20 | 253 |
| Primer 2 details |
|---|
| Sequence | TCCTCCTAGAACTCCACCGG | ACGACCACGAGCATGAACAT | Start: 1630 |
| Tm | 60.033 | 60.037 | End: 1883 |
| Size | 20 | 20 | 254 |
| Primer 3 details |
|---|
| Sequence | TCCATGCGATTGTTGCTCCT | ACGACCACGAGCATGAACAT | Start: 1614 |
| Tm | 60.035 | 60.037 | End: 1883 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
|---|
| Sequence | GTTTTCCTCCGCCGAAAACC | ACGACCACGAGCATGAACAT | Start: 1686 |
| Tm | 60.04 | 60.037 | End: 1883 |
| Size | 20 | 20 | 198 |
| Primer 5 details |
|---|
| Sequence | AATCTCTACCACTGGGCCCT | CCGGTGGAGTTCTAGGAGGA | Start: 1450 |
| Tm | 59.955 | 60.033 | End: 1649 |
| Size | 20 | 20 | 200 |
(A)14caacgacttacagaaatcctagaagaagtaaaaagaaaaacggatccaaaatcag(A)11cgaaaatcctcgctcttgccgtttcttccgcgcagagaaatccacaacactagtaaggttgattattccccccagagcgcgctccctctaccccttca(TC)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCCTAGAACTCCACCGGA | ACGACCACGAGCATGAACAT | Start: 1631 |
| Tm | 60.033 | 60.037 | End: 1883 |
| Size | 20 | 20 | 253 |
| Primer 2 details |
|---|
| Sequence | TCCTCCTAGAACTCCACCGG | ACGACCACGAGCATGAACAT | Start: 1630 |
| Tm | 60.033 | 60.037 | End: 1883 |
| Size | 20 | 20 | 254 |
| Primer 3 details |
|---|
| Sequence | TCCATGCGATTGTTGCTCCT | ACGACCACGAGCATGAACAT | Start: 1614 |
| Tm | 60.035 | 60.037 | End: 1883 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
|---|
| Sequence | GTTTTCCTCCGCCGAAAACC | ACGACCACGAGCATGAACAT | Start: 1686 |
| Tm | 60.04 | 60.037 | End: 1883 |
| Size | 20 | 20 | 198 |
| Primer 5 details |
|---|
| Sequence | AATCTCTACCACTGGGCCCT | CCGGTGGAGTTCTAGGAGGA | Start: 1450 |
| Tm | 59.955 | 60.033 | End: 1649 |
| Size | 20 | 20 | 200 |
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