ssr_primer
Primer Details for TRINITY_DN13882_c2_g6_i3 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCTTCCTTGCGAGAAAAGC | CTGCCTCGTTTCAGGGGAAT | Start: 999 |
| Tm | 60.11 | 60.035 | End: 1196 |
| Size | 20 | 20 | 198 |
| Primer 2 details |
|---|
| Sequence | GAAAGGCCCACAGAACTGGA | CAGCAAGCAGGCAAAAGAGG | Start: 7 |
| Tm | 59.89 | 60.039 | End: 249 |
| Size | 20 | 20 | 243 |
| Primer 3 details |
|---|
| Sequence | AAGGCCCACAGAACTGGAAG | CAGCAAGCAGGCAAAAGAGG | Start: 9 |
| Tm | 59.889 | 60.039 | End: 249 |
| Size | 20 | 20 | 241 |
| Primer 4 details |
|---|
| Sequence | CCCCACTGGTACCAACGTAC | TGCTTGATCTTGTTGGCCCT | Start: 1669 |
| Tm | 60.037 | 59.887 | End: 1923 |
| Size | 20 | 20 | 255 |
| Primer 5 details |
|---|
| Sequence | ACATGGCTGCCACTGAATCA | TGCTTGATCTTGTTGGCCCT | Start: 1687 |
| Tm | 59.961 | 59.887 | End: 1923 |
| Size | 20 | 20 | 237 |
(T)20atgtaattggggttgaattttggagggcccttgattcatttttcgtgttacttggataac(T)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCTTCCTTGCGAGAAAAGC | CTGCCTCGTTTCAGGGGAAT | Start: 999 |
| Tm | 60.11 | 60.035 | End: 1196 |
| Size | 20 | 20 | 198 |
| Primer 2 details |
|---|
| Sequence | GAAAGGCCCACAGAACTGGA | CAGCAAGCAGGCAAAAGAGG | Start: 7 |
| Tm | 59.89 | 60.039 | End: 249 |
| Size | 20 | 20 | 243 |
| Primer 3 details |
|---|
| Sequence | AAGGCCCACAGAACTGGAAG | CAGCAAGCAGGCAAAAGAGG | Start: 9 |
| Tm | 59.889 | 60.039 | End: 249 |
| Size | 20 | 20 | 241 |
| Primer 4 details |
|---|
| Sequence | CCCCACTGGTACCAACGTAC | TGCTTGATCTTGTTGGCCCT | Start: 1669 |
| Tm | 60.037 | 59.887 | End: 1923 |
| Size | 20 | 20 | 255 |
| Primer 5 details |
|---|
| Sequence | ACATGGCTGCCACTGAATCA | TGCTTGATCTTGTTGGCCCT | Start: 1687 |
| Tm | 59.961 | 59.887 | End: 1923 |
| Size | 20 | 20 | 237 |
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