ssr_primer
Primer Details for TRINITY_DN13802_c1_g1_i5 |
|---|
| (A)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTATGCGCACACCGTATCCT | ACCTAACCCCAAAGATGGCG | Start: 4848 |
| Tm | 59.968 | 60.034 | End: 4966 |
| Size | 20 | 20 | 119 |
| Primer 2 details |
|---|
| Sequence | CTGCATCTTGACGACCCACT | AGGATACGGTGTGCGCATAG | Start: 4619 |
| Tm | 60.037 | 59.968 | End: 4867 |
| Size | 20 | 20 | 249 |
| Primer 3 details |
|---|
| Sequence | GCATCTTGACGACCCACTGA | AGGATACGGTGTGCGCATAG | Start: 4621 |
| Tm | 60.037 | 59.968 | End: 4867 |
| Size | 20 | 20 | 247 |
| Primer 4 details |
|---|
| Sequence | AAAATCCGCTGCTGGGTACA | AGGATACGGTGTGCGCATAG | Start: 4644 |
| Tm | 59.962 | 59.968 | End: 4867 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | ACCCCTTCAGCAAGCTTCTC | CTGTGCTCACCCTTCGAACT | Start: 2385 |
| Tm | 59.963 | 59.966 | End: 2621 |
| Size | 20 | 20 | 237 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTATGCGCACACCGTATCCT | ACCTAACCCCAAAGATGGCG | Start: 4848 |
| Tm | 59.968 | 60.034 | End: 4966 |
| Size | 20 | 20 | 119 |
| Primer 2 details |
|---|
| Sequence | CTGCATCTTGACGACCCACT | AGGATACGGTGTGCGCATAG | Start: 4619 |
| Tm | 60.037 | 59.968 | End: 4867 |
| Size | 20 | 20 | 249 |
| Primer 3 details |
|---|
| Sequence | GCATCTTGACGACCCACTGA | AGGATACGGTGTGCGCATAG | Start: 4621 |
| Tm | 60.037 | 59.968 | End: 4867 |
| Size | 20 | 20 | 247 |
| Primer 4 details |
|---|
| Sequence | AAAATCCGCTGCTGGGTACA | AGGATACGGTGTGCGCATAG | Start: 4644 |
| Tm | 59.962 | 59.968 | End: 4867 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | ACCCCTTCAGCAAGCTTCTC | CTGTGCTCACCCTTCGAACT | Start: 2385 |
| Tm | 59.963 | 59.966 | End: 2621 |
| Size | 20 | 20 | 237 |
(T)13(G)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTATGCGCACACCGTATCCT | ACCTAACCCCAAAGATGGCG | Start: 4848 |
| Tm | 59.968 | 60.034 | End: 4966 |
| Size | 20 | 20 | 119 |
| Primer 2 details |
|---|
| Sequence | CTGCATCTTGACGACCCACT | AGGATACGGTGTGCGCATAG | Start: 4619 |
| Tm | 60.037 | 59.968 | End: 4867 |
| Size | 20 | 20 | 249 |
| Primer 3 details |
|---|
| Sequence | GCATCTTGACGACCCACTGA | AGGATACGGTGTGCGCATAG | Start: 4621 |
| Tm | 60.037 | 59.968 | End: 4867 |
| Size | 20 | 20 | 247 |
| Primer 4 details |
|---|
| Sequence | AAAATCCGCTGCTGGGTACA | AGGATACGGTGTGCGCATAG | Start: 4644 |
| Tm | 59.962 | 59.968 | End: 4867 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | ACCCCTTCAGCAAGCTTCTC | CTGTGCTCACCCTTCGAACT | Start: 2385 |
| Tm | 59.963 | 59.966 | End: 2621 |
| Size | 20 | 20 | 237 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTATGCGCACACCGTATCCT | ACCTAACCCCAAAGATGGCG | Start: 4848 |
| Tm | 59.968 | 60.034 | End: 4966 |
| Size | 20 | 20 | 119 |
| Primer 2 details |
|---|
| Sequence | CTGCATCTTGACGACCCACT | AGGATACGGTGTGCGCATAG | Start: 4619 |
| Tm | 60.037 | 59.968 | End: 4867 |
| Size | 20 | 20 | 249 |
| Primer 3 details |
|---|
| Sequence | GCATCTTGACGACCCACTGA | AGGATACGGTGTGCGCATAG | Start: 4621 |
| Tm | 60.037 | 59.968 | End: 4867 |
| Size | 20 | 20 | 247 |
| Primer 4 details |
|---|
| Sequence | AAAATCCGCTGCTGGGTACA | AGGATACGGTGTGCGCATAG | Start: 4644 |
| Tm | 59.962 | 59.968 | End: 4867 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | ACCCCTTCAGCAAGCTTCTC | CTGTGCTCACCCTTCGAACT | Start: 2385 |
| Tm | 59.963 | 59.966 | End: 2621 |
| Size | 20 | 20 | 237 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTATGCGCACACCGTATCCT | ACCTAACCCCAAAGATGGCG | Start: 4848 |
| Tm | 59.968 | 60.034 | End: 4966 |
| Size | 20 | 20 | 119 |
| Primer 2 details |
|---|
| Sequence | CTGCATCTTGACGACCCACT | AGGATACGGTGTGCGCATAG | Start: 4619 |
| Tm | 60.037 | 59.968 | End: 4867 |
| Size | 20 | 20 | 249 |
| Primer 3 details |
|---|
| Sequence | GCATCTTGACGACCCACTGA | AGGATACGGTGTGCGCATAG | Start: 4621 |
| Tm | 60.037 | 59.968 | End: 4867 |
| Size | 20 | 20 | 247 |
| Primer 4 details |
|---|
| Sequence | AAAATCCGCTGCTGGGTACA | AGGATACGGTGTGCGCATAG | Start: 4644 |
| Tm | 59.962 | 59.968 | End: 4867 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | ACCCCTTCAGCAAGCTTCTC | CTGTGCTCACCCTTCGAACT | Start: 2385 |
| Tm | 59.963 | 59.966 | End: 2621 |
| Size | 20 | 20 | 237 |
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