ssr_primer
Primer Details for TRINITY_DN13714_c1_g2_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATTCCGAGCTCCACCATGG | TGCTTTTGAGTACGACGGCT | Start: 1020 |
| Tm | 60.107 | 59.967 | End: 1238 |
| Size | 20 | 20 | 219 |
| Primer 2 details |
|---|
| Sequence | GCAAACGTGGGCAGAGAAAG | CCATGGTGGAGCTCGGAATT | Start: 817 |
| Tm | 60.04 | 60.107 | End: 1039 |
| Size | 20 | 20 | 223 |
| Primer 3 details |
|---|
| Sequence | CCTGAGTTTTCGGGTCAGCT | TGTTGGCCACCTACCTAGGA | Start: 127 |
| Tm | 59.965 | 59.882 | End: 247 |
| Size | 20 | 20 | 121 |
| Primer 4 details |
|---|
| Sequence | GTAGGGACACTGAGCAGTCG | CTTTCTCTGCCCACGTTTGC | Start: 737 |
| Tm | 59.829 | 60.04 | End: 836 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
|---|
| Sequence | TGCGGGTGGTTCTCTTGTTT | CCATGGTGGAGCTCGGAATT | Start: 879 |
| Tm | 60.107 | 60.107 | End: 1039 |
| Size | 20 | 20 | 161 |
(T)18: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATTCCGAGCTCCACCATGG | TGCTTTTGAGTACGACGGCT | Start: 1020 |
| Tm | 60.107 | 59.967 | End: 1238 |
| Size | 20 | 20 | 219 |
| Primer 2 details |
|---|
| Sequence | GCAAACGTGGGCAGAGAAAG | CCATGGTGGAGCTCGGAATT | Start: 817 |
| Tm | 60.04 | 60.107 | End: 1039 |
| Size | 20 | 20 | 223 |
| Primer 3 details |
|---|
| Sequence | CCTGAGTTTTCGGGTCAGCT | TGTTGGCCACCTACCTAGGA | Start: 127 |
| Tm | 59.965 | 59.882 | End: 247 |
| Size | 20 | 20 | 121 |
| Primer 4 details |
|---|
| Sequence | GTAGGGACACTGAGCAGTCG | CTTTCTCTGCCCACGTTTGC | Start: 737 |
| Tm | 59.829 | 60.04 | End: 836 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
|---|
| Sequence | TGCGGGTGGTTCTCTTGTTT | CCATGGTGGAGCTCGGAATT | Start: 879 |
| Tm | 60.107 | 60.107 | End: 1039 |
| Size | 20 | 20 | 161 |
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