ssr_primer
Primer Details for TRINITY_DN13451_c0_g7_i2 |
|---|
| (A)18: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCTTGGTGCCCTTCAAAAG | GGAGGGTGGAGATGAGGACT | Start: 1059 |
| Tm | 59.966 | 60.032 | End: 1336 |
| Size | 20 | 20 | 278 |
| Primer 2 details |
|---|
| Sequence | AGTCCTCATCTCCACCCTCC | TTCCCTACTGCTGCCCATTG | Start: 1317 |
| Tm | 60.032 | 60.034 | End: 1451 |
| Size | 20 | 20 | 135 |
| Primer 3 details |
|---|
| Sequence | AGTCCTCATCTCCACCCTCC | GCCCATTGCCATGCCATTAG | Start: 1317 |
| Tm | 60.032 | 59.966 | End: 1439 |
| Size | 20 | 20 | 123 |
| Primer 4 details |
|---|
| Sequence | CAACAGAGACTGCCAGCTCA | GGAGGGTGGAGATGAGGACT | Start: 1129 |
| Tm | 59.966 | 60.032 | End: 1336 |
| Size | 20 | 20 | 208 |
| Primer 5 details |
|---|
| Sequence | GCCTTGGTGCCCTTCAAAAG | GCATGTTCTCTTGGTCGGGA | Start: 1059 |
| Tm | 59.966 | 60.036 | End: 1280 |
| Size | 20 | 20 | 222 |
(T)11gtttgacaaggagagagggtacccttgtcggggtcacgagaagctattagacagaaacgaataggaatgggttgattggaggaggggc(T)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCTTGGTGCCCTTCAAAAG | GGAGGGTGGAGATGAGGACT | Start: 1059 |
| Tm | 59.966 | 60.032 | End: 1336 |
| Size | 20 | 20 | 278 |
| Primer 2 details |
|---|
| Sequence | AGTCCTCATCTCCACCCTCC | TTCCCTACTGCTGCCCATTG | Start: 1317 |
| Tm | 60.032 | 60.034 | End: 1451 |
| Size | 20 | 20 | 135 |
| Primer 3 details |
|---|
| Sequence | AGTCCTCATCTCCACCCTCC | GCCCATTGCCATGCCATTAG | Start: 1317 |
| Tm | 60.032 | 59.966 | End: 1439 |
| Size | 20 | 20 | 123 |
| Primer 4 details |
|---|
| Sequence | CAACAGAGACTGCCAGCTCA | GGAGGGTGGAGATGAGGACT | Start: 1129 |
| Tm | 59.966 | 60.032 | End: 1336 |
| Size | 20 | 20 | 208 |
| Primer 5 details |
|---|
| Sequence | GCCTTGGTGCCCTTCAAAAG | GCATGTTCTCTTGGTCGGGA | Start: 1059 |
| Tm | 59.966 | 60.036 | End: 1280 |
| Size | 20 | 20 | 222 |
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