ssr_primer
Primer Details for TRINITY_DN13336_c5_g3_i2 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACACCGAGTTCCCGATCAG | CCGAACATCGCGAACAATCC | Start: 1169 |
| Tm | 59.753 | 59.974 | End: 1290 |
| Size | 20 | 20 | 122 |
| Primer 2 details |
|---|
| Sequence | AACACCGAGTTCCCGATCAG | CGTGCCCTCTTCTCCCATTT | Start: 1169 |
| Tm | 59.753 | 60.035 | End: 1328 |
| Size | 20 | 20 | 160 |
| Primer 3 details |
|---|
| Sequence | GGTCGTGACCCTTTCTTCGT | CGTGAGCGTTACAGTGGGAA | Start: 310 |
| Tm | 59.967 | 60.319 | End: 581 |
| Size | 20 | 20 | 272 |
| Primer 4 details |
|---|
| Sequence | GGTCGTGACCCTTTCTTCGT | TTCGTGAGCGTTACAGTGGG | Start: 310 |
| Tm | 59.967 | 60.319 | End: 583 |
| Size | 20 | 20 | 274 |
| Primer 5 details |
|---|
| Sequence | CCCACTGTAACGCTCACGAA | CGTTCACATGGAGGCTGAGT | Start: 564 |
| Tm | 60.319 | 60.037 | End: 663 |
| Size | 20 | 20 | 100 |
(CT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACACCGAGTTCCCGATCAG | CCGAACATCGCGAACAATCC | Start: 1169 |
| Tm | 59.753 | 59.974 | End: 1290 |
| Size | 20 | 20 | 122 |
| Primer 2 details |
|---|
| Sequence | AACACCGAGTTCCCGATCAG | CGTGCCCTCTTCTCCCATTT | Start: 1169 |
| Tm | 59.753 | 60.035 | End: 1328 |
| Size | 20 | 20 | 160 |
| Primer 3 details |
|---|
| Sequence | GGTCGTGACCCTTTCTTCGT | CGTGAGCGTTACAGTGGGAA | Start: 310 |
| Tm | 59.967 | 60.319 | End: 581 |
| Size | 20 | 20 | 272 |
| Primer 4 details |
|---|
| Sequence | GGTCGTGACCCTTTCTTCGT | TTCGTGAGCGTTACAGTGGG | Start: 310 |
| Tm | 59.967 | 60.319 | End: 583 |
| Size | 20 | 20 | 274 |
| Primer 5 details |
|---|
| Sequence | CCCACTGTAACGCTCACGAA | CGTTCACATGGAGGCTGAGT | Start: 564 |
| Tm | 60.319 | 60.037 | End: 663 |
| Size | 20 | 20 | 100 |
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