Primer Details for TRINITY_DN13313_c4_g7_i1 |
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| (AT)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAACACGATGGGATGGAGCA | TCATTTTTGGTCAATGTATGGATAGT | Start: 38 |
| Tm | 60.108 | 57.178 | End: 281 |
| Size | 20 | 26 | 244 |
| Primer 2 details |
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| Sequence | AGAACACGATGGGATGGAGC | TCATTTTTGGTCAATGTATGGATAGT | Start: 37 |
| Tm | 59.821 | 57.178 | End: 281 |
| Size | 20 | 26 | 245 |
| Primer 3 details |
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| Sequence | AACACGATGGGATGGAGCAA | TCATTTTTGGTCAATGTATGGATAGT | Start: 39 |
| Tm | 59.672 | 57.178 | End: 281 |
| Size | 20 | 26 | 243 |
| Primer 4 details |
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| Sequence | ACACGATGGGATGGAGCAAC | TCATTTTTGGTCAATGTATGGATAGT | Start: 40 |
| Tm | 60.393 | 57.178 | End: 281 |
| Size | 20 | 26 | 242 |
| Primer 5 details |
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| Sequence | CGATGGGATGGAGCAACGAA | TCATTTTTGGTCAATGTATGGATAGT | Start: 43 |
| Tm | 60.462 | 57.178 | End: 281 |
| Size | 20 | 26 | 239 |
