ssr_primer
Primer Details for TRINITY_DN13247_c4_g1_i1 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAGAACAGCAACAGGAGGT | TTGGTCCTTGGTGAAGCTCC | Start: 1001 |
| Tm | 59.891 | 59.89 | End: 1266 |
| Size | 20 | 20 | 266 |
| Primer 2 details |
|---|
| Sequence | TCCCACAGAAGGAAGGGACT | TGCACTGGTTTGTGTGGTCT | Start: 402 |
| Tm | 59.806 | 60.035 | End: 588 |
| Size | 20 | 20 | 187 |
| Primer 3 details |
|---|
| Sequence | TCCCACAGAAGGAAGGGACT | TCTGCACTGGTTTGTGTGGT | Start: 402 |
| Tm | 59.806 | 60.035 | End: 590 |
| Size | 20 | 20 | 189 |
| Primer 4 details |
|---|
| Sequence | CAGGGAAGCACATACACCGT | TCCCTTCCTTCTGTGGGACT | Start: 173 |
| Tm | 60.036 | 59.806 | End: 419 |
| Size | 20 | 20 | 247 |
| Primer 5 details |
|---|
| Sequence | CAGGGAAGCACATACACCGT | AGTCCCTTCCTTCTGTGGGA | Start: 173 |
| Tm | 60.036 | 59.806 | End: 421 |
| Size | 20 | 20 | 249 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAGAACAGCAACAGGAGGT | TTGGTCCTTGGTGAAGCTCC | Start: 1001 |
| Tm | 59.891 | 59.89 | End: 1266 |
| Size | 20 | 20 | 266 |
| Primer 2 details |
|---|
| Sequence | TCCCACAGAAGGAAGGGACT | TGCACTGGTTTGTGTGGTCT | Start: 402 |
| Tm | 59.806 | 60.035 | End: 588 |
| Size | 20 | 20 | 187 |
| Primer 3 details |
|---|
| Sequence | TCCCACAGAAGGAAGGGACT | TCTGCACTGGTTTGTGTGGT | Start: 402 |
| Tm | 59.806 | 60.035 | End: 590 |
| Size | 20 | 20 | 189 |
| Primer 4 details |
|---|
| Sequence | CAGGGAAGCACATACACCGT | TCCCTTCCTTCTGTGGGACT | Start: 173 |
| Tm | 60.036 | 59.806 | End: 419 |
| Size | 20 | 20 | 247 |
| Primer 5 details |
|---|
| Sequence | CAGGGAAGCACATACACCGT | AGTCCCTTCCTTCTGTGGGA | Start: 173 |
| Tm | 60.036 | 59.806 | End: 421 |
| Size | 20 | 20 | 249 |
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