ssr_primer
Primer Details for TRINITY_DN13219_c1_g1_i1 |
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| (T)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAGGAAAATGGGGTTGTGA | ACTGACTGACTGCACACTCC | Start: 1163 |
| Tm | 59.214 | 59.609 | End: 1296 |
| Size | 21 | 20 | 134 |
| Primer 2 details |
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| Sequence | AGGAGGAAAATGGGGTTGTGA | CTGACTGACTGCACACTCCT | Start: 1163 |
| Tm | 59.214 | 59.322 | End: 1295 |
| Size | 21 | 20 | 133 |
| Primer 3 details |
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| Sequence | GGAGGAAAATGGGGTTGTGAG | ACTGACTGACTGCACACTCC | Start: 1164 |
| Tm | 58.829 | 59.609 | End: 1296 |
| Size | 21 | 20 | 133 |
| Primer 4 details |
|---|
| Sequence | AAGGAGGAAAATGGGGTTGTGA | ACTGACTGACTGCACACTCC | Start: 1162 |
| Tm | 59.821 | 59.609 | End: 1296 |
| Size | 22 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | GGAGGAAAATGGGGTTGTGAGA | ACTGACTGACTGCACACTCC | Start: 1164 |
| Tm | 60.225 | 59.609 | End: 1296 |
| Size | 22 | 20 | 133 |
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