ssr_primer
Primer Details for TRINITY_DN12954_c0_g5_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGAACGTTATCAAACACCCC | TCACGGTACTTGCAAGTGGA | Start: 322 |
| Tm | 59.186 | 59.246 | End: 477 |
| Size | 21 | 20 | 156 |
| Primer 2 details |
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| Sequence | CACGTGGGAAAATGGAAAAGGA | TGCTCTCGTTCTCCGTTTCC | Start: 147 |
| Tm | 59.374 | 60.039 | End: 391 |
| Size | 22 | 20 | 245 |
| Primer 3 details |
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| Sequence | TCACGTGGGAAAATGGAAAAGG | TGCTCTCGTTCTCCGTTTCC | Start: 146 |
| Tm | 59.374 | 60.039 | End: 391 |
| Size | 22 | 20 | 246 |
| Primer 4 details |
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| Sequence | GGAAATCACGTGGGAAAATGGA | TGCTCTCGTTCTCCGTTTCC | Start: 141 |
| Tm | 59.177 | 60.039 | End: 391 |
| Size | 22 | 20 | 251 |
| Primer 5 details |
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| Sequence | AGGAAATCACGTGGGAAAATGG | TGCTCTCGTTCTCCGTTTCC | Start: 140 |
| Tm | 59.174 | 60.039 | End: 391 |
| Size | 22 | 20 | 252 |
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