ssr_primers

ssr_primer

Primer Details for TRINITY_DN12904_c5_g5_i6
(GAGAGG)7: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	TCTGGTCGTGCCCATTATCG	CCAGAGAGCCCACAACTCAG	Start: 949
Tm	59.896	60.037	End: 1227
Size	20	20	279
Primer 2 details
Sequence	AACCATCCGATTGGCCTGAG	CCAGAGAGCCCACAACTCAG	Start: 993
Tm	60.107	60.037	End: 1227
Size	20	20	235
Primer 3 details
Sequence	GAACCATCCGATTGGCCTGA	CCAGAGAGCCCACAACTCAG	Start: 992
Tm	60.107	60.037	End: 1227
Size	20	20	236
Primer 4 details
Sequence	TGGACGGTTGTGATACACCG	TTGTTCGGTGGAGCAACACT	Start: 2549
Tm	60.038	60.107	End: 2674
Size	20	20	126
Primer 5 details
Sequence	TGGACGGTTGTGATACACCG	ACAACAGCTTGTTCGGTGGA	Start: 2549
Tm	60.038	60.107	End: 2682
Size	20	20	134
(AC)9aaacaa(AAT)6: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	TCTGGTCGTGCCCATTATCG	CCAGAGAGCCCACAACTCAG	Start: 949
Tm	59.896	60.037	End: 1227
Size	20	20	279
Primer 2 details
Sequence	AACCATCCGATTGGCCTGAG	CCAGAGAGCCCACAACTCAG	Start: 993
Tm	60.107	60.037	End: 1227
Size	20	20	235
Primer 3 details
Sequence	GAACCATCCGATTGGCCTGA	CCAGAGAGCCCACAACTCAG	Start: 992
Tm	60.107	60.037	End: 1227
Size	20	20	236
Primer 4 details
Sequence	TGGACGGTTGTGATACACCG	TTGTTCGGTGGAGCAACACT	Start: 2549
Tm	60.038	60.107	End: 2674
Size	20	20	126
Primer 5 details
Sequence	TGGACGGTTGTGATACACCG	ACAACAGCTTGTTCGGTGGA	Start: 2549
Tm	60.038	60.107	End: 2682
Size	20	20	134
(A)10: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	TCTGGTCGTGCCCATTATCG	CCAGAGAGCCCACAACTCAG	Start: 949
Tm	59.896	60.037	End: 1227
Size	20	20	279
Primer 2 details
Sequence	AACCATCCGATTGGCCTGAG	CCAGAGAGCCCACAACTCAG	Start: 993
Tm	60.107	60.037	End: 1227
Size	20	20	235
Primer 3 details
Sequence	GAACCATCCGATTGGCCTGA	CCAGAGAGCCCACAACTCAG	Start: 992
Tm	60.107	60.037	End: 1227
Size	20	20	236
Primer 4 details
Sequence	TGGACGGTTGTGATACACCG	TTGTTCGGTGGAGCAACACT	Start: 2549
Tm	60.038	60.107	End: 2674
Size	20	20	126
Primer 5 details
Sequence	TGGACGGTTGTGATACACCG	ACAACAGCTTGTTCGGTGGA	Start: 2549
Tm	60.038	60.107	End: 2682
Size	20	20	134
(AGA)5: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	TCTGGTCGTGCCCATTATCG	CCAGAGAGCCCACAACTCAG	Start: 949
Tm	59.896	60.037	End: 1227
Size	20	20	279
Primer 2 details
Sequence	AACCATCCGATTGGCCTGAG	CCAGAGAGCCCACAACTCAG	Start: 993
Tm	60.107	60.037	End: 1227
Size	20	20	235
Primer 3 details
Sequence	GAACCATCCGATTGGCCTGA	CCAGAGAGCCCACAACTCAG	Start: 992
Tm	60.107	60.037	End: 1227
Size	20	20	236
Primer 4 details
Sequence	TGGACGGTTGTGATACACCG	TTGTTCGGTGGAGCAACACT	Start: 2549
Tm	60.038	60.107	End: 2674
Size	20	20	126
Primer 5 details
Sequence	TGGACGGTTGTGATACACCG	ACAACAGCTTGTTCGGTGGA	Start: 2549
Tm	60.038	60.107	End: 2682
Size	20	20	134
(TGT)6: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	TCTGGTCGTGCCCATTATCG	CCAGAGAGCCCACAACTCAG	Start: 949
Tm	59.896	60.037	End: 1227
Size	20	20	279
Primer 2 details
Sequence	AACCATCCGATTGGCCTGAG	CCAGAGAGCCCACAACTCAG	Start: 993
Tm	60.107	60.037	End: 1227
Size	20	20	235
Primer 3 details
Sequence	GAACCATCCGATTGGCCTGA	CCAGAGAGCCCACAACTCAG	Start: 992
Tm	60.107	60.037	End: 1227
Size	20	20	236
Primer 4 details
Sequence	TGGACGGTTGTGATACACCG	TTGTTCGGTGGAGCAACACT	Start: 2549
Tm	60.038	60.107	End: 2674
Size	20	20	126
Primer 5 details
Sequence	TGGACGGTTGTGATACACCG	ACAACAGCTTGTTCGGTGGA	Start: 2549
Tm	60.038	60.107	End: 2682
Size	20	20	134
(GAA)5: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	TCTGGTCGTGCCCATTATCG	CCAGAGAGCCCACAACTCAG	Start: 949
Tm	59.896	60.037	End: 1227
Size	20	20	279
Primer 2 details
Sequence	AACCATCCGATTGGCCTGAG	CCAGAGAGCCCACAACTCAG	Start: 993
Tm	60.107	60.037	End: 1227
Size	20	20	235
Primer 3 details
Sequence	GAACCATCCGATTGGCCTGA	CCAGAGAGCCCACAACTCAG	Start: 992
Tm	60.107	60.037	End: 1227
Size	20	20	236
Primer 4 details
Sequence	TGGACGGTTGTGATACACCG	TTGTTCGGTGGAGCAACACT	Start: 2549
Tm	60.038	60.107	End: 2674
Size	20	20	126
Primer 5 details
Sequence	TGGACGGTTGTGATACACCG	ACAACAGCTTGTTCGGTGGA	Start: 2549
Tm	60.038	60.107	End: 2682
Size	20	20	134
(T)16: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	TCTGGTCGTGCCCATTATCG	CCAGAGAGCCCACAACTCAG	Start: 949
Tm	59.896	60.037	End: 1227
Size	20	20	279
Primer 2 details
Sequence	AACCATCCGATTGGCCTGAG	CCAGAGAGCCCACAACTCAG	Start: 993
Tm	60.107	60.037	End: 1227
Size	20	20	235
Primer 3 details
Sequence	GAACCATCCGATTGGCCTGA	CCAGAGAGCCCACAACTCAG	Start: 992
Tm	60.107	60.037	End: 1227
Size	20	20	236
Primer 4 details
Sequence	TGGACGGTTGTGATACACCG	TTGTTCGGTGGAGCAACACT	Start: 2549
Tm	60.038	60.107	End: 2674
Size	20	20	126
Primer 5 details
Sequence	TGGACGGTTGTGATACACCG	ACAACAGCTTGTTCGGTGGA	Start: 2549
Tm	60.038	60.107	End: 2682
Size	20	20	134
(GAA)5: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	TCTGGTCGTGCCCATTATCG	CCAGAGAGCCCACAACTCAG	Start: 949
Tm	59.896	60.037	End: 1227
Size	20	20	279
Primer 2 details
Sequence	AACCATCCGATTGGCCTGAG	CCAGAGAGCCCACAACTCAG	Start: 993
Tm	60.107	60.037	End: 1227
Size	20	20	235
Primer 3 details
Sequence	GAACCATCCGATTGGCCTGA	CCAGAGAGCCCACAACTCAG	Start: 992
Tm	60.107	60.037	End: 1227
Size	20	20	236
Primer 4 details
Sequence	TGGACGGTTGTGATACACCG	TTGTTCGGTGGAGCAACACT	Start: 2549
Tm	60.038	60.107	End: 2674
Size	20	20	126
Primer 5 details
Sequence	TGGACGGTTGTGATACACCG	ACAACAGCTTGTTCGGTGGA	Start: 2549
Tm	60.038	60.107	End: 2682
Size	20	20	134

CsExSLDb: Transcriptome based Cucumis sativus Extended Shelf Life Database

Primer Details for TRINITY_DN12904_c5_g5_i6

ICAR Data Use Licence