Primer Details for TRINITY_DN12898_c6_g4_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAAGGAAAAAGAAGATTCGAGTAGA | TGGGTTTTATATGGACTCTTCTTTCA | Start: 54 |
| Tm | 57.096 | 58.305 | End: 153 |
| Size | 26 | 26 | 100 |
| Primer 2 details |
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| Sequence | AAAAGGAAAAAGAAGATTCGAGTAGAA | TGGGTTTTATATGGACTCTTCTTTCA | Start: 54 |
| Tm | 57.661 | 58.305 | End: 153 |
| Size | 27 | 26 | 100 |
| Primer 3 details |
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| Sequence | AAAAAGGAAAAAGAAGATTCGAGTAGA | TGGGTTTTATATGGACTCTTCTTTCA | Start: 53 |
| Tm | 57.661 | 58.305 | End: 153 |
| Size | 27 | 26 | 101 |
| Primer 4 details |
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| Sequence | AAAGGAAAAAGAAGATTCGAGTAGAA | TTGGGTTTTATATGGACTCTTCTTTCA | Start: 55 |
| Tm | 57.096 | 58.833 | End: 154 |
| Size | 26 | 27 | 100 |
| Primer 5 details |
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| Sequence | AAAAGGAAAAAGAAGATTCGAGTAGA | TGGGTTTTATATGGACTCTTCTTTCAA | Start: 54 |
| Tm | 57.096 | 58.833 | End: 153 |
| Size | 26 | 27 | 100 |
