Primer Details for TRINITY_DN12888_c0_g1_i1 | |||
|---|---|---|---|
| (A)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCAAGGTACAATTATGGAATCGT | GCACCTCTTATCAAGCTTGAAGC | Start: 182 |
| Tm | 59.421 | 60.182 | End: 332 |
| Size | 24 | 23 | 151 |
| Primer 2 details | |||
| Sequence | AGGCAAGGTACAATTATGGAATCG | GCACCTCTTATCAAGCTTGAAGC | Start: 181 |
| Tm | 59.178 | 60.182 | End: 332 |
| Size | 24 | 23 | 152 |
| Primer 3 details | |||
| Sequence | GGCAAGGTACAATTATGGAATCGT | TGCACCTCTTATCAAGCTTGA | Start: 182 |
| Tm | 59.421 | 57.299 | End: 333 |
| Size | 24 | 21 | 152 |
| Primer 4 details | |||
| Sequence | GGCAAGGTACAATTATGGAATCG | GCACCTCTTATCAAGCTTGAAGC | Start: 182 |
| Tm | 57.892 | 60.182 | End: 332 |
| Size | 23 | 23 | 151 |
| Primer 5 details | |||
| Sequence | AGGTACAATTATGGAATCGTGAGGT | GCACCTCTTATCAAGCTTGAAGC | Start: 186 |
| Tm | 59.87 | 60.182 | End: 332 |
| Size | 25 | 23 | 147 | (AAT)6: Primer 1 details |
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCAAGGTACAATTATGGAATCGT | GCACCTCTTATCAAGCTTGAAGC | Start: 182 |
| Tm | 59.421 | 60.182 | End: 332 |
| Size | 24 | 23 | 151 |
| Primer 2 details | |||
| Sequence | AGGCAAGGTACAATTATGGAATCG | GCACCTCTTATCAAGCTTGAAGC | Start: 181 |
| Tm | 59.178 | 60.182 | End: 332 |
| Size | 24 | 23 | 152 |
| Primer 3 details | |||
| Sequence | GGCAAGGTACAATTATGGAATCGT | TGCACCTCTTATCAAGCTTGA | Start: 182 |
| Tm | 59.421 | 57.299 | End: 333 |
| Size | 24 | 21 | 152 |
| Primer 4 details | |||
| Sequence | GGCAAGGTACAATTATGGAATCG | GCACCTCTTATCAAGCTTGAAGC | Start: 182 |
| Tm | 57.892 | 60.182 | End: 332 |
| Size | 23 | 23 | 151 |
| Primer 5 details | |||
| Sequence | AGGTACAATTATGGAATCGTGAGGT | GCACCTCTTATCAAGCTTGAAGC | Start: 186 |
| Tm | 59.87 | 60.182 | End: 332 |
| Size | 25 | 23 | 147 |
