ssr_primer
Primer Details for TRINITY_DN12646_c2_g2_i5 |
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| (TCTCCT)5tcgtggacttgacttcgtggcgattagaaccatctactcccattacatcaccctctcattttcaaatagcaccgtccgcagaaactgtcatccaatt(TTC)7: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGATTCCATGGGGATGGGGA | GCATTGAGCAAGCCCATAGC | Start: 243 |
| Tm | 60.028 | 59.968 | End: 522 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
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| Sequence | GCTATGGGCTTGCTCAATGC | GTTCCCGCAAATTGCAGTGT | Start: 503 |
| Tm | 59.968 | 59.968 | End: 702 |
| Size | 20 | 20 | 200 |
| Primer 3 details |
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| Sequence | GCTATGGGCTTGCTCAATGC | AACCTCAGGAAGCAGCCATC | Start: 503 |
| Tm | 59.968 | 60.035 | End: 641 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
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| Sequence | GCTATGGGCTTGCTCAATGC | GAACCTCAGGAAGCAGCCAT | Start: 503 |
| Tm | 59.968 | 60.035 | End: 642 |
| Size | 20 | 20 | 140 |
| Primer 5 details |
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| Sequence | AGAAAAGAGAGGAAGCCGCC | CCACGAAGTCAAGTCCACGA | Start: 13 |
| Tm | 60.036 | 59.968 | End: 117 |
| Size | 20 | 20 | 105 |
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