ssr_primer
Primer Details for TRINITY_DN12434_c1_g4_i2 |
|---|
| (A)12g(A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGGGGAATTATAGCGCCG | ACGTCCCTCATCCTCACTCA | Start: 3095 |
| Tm | 59.965 | 59.96 | End: 3345 |
| Size | 20 | 20 | 251 |
| Primer 2 details |
|---|
| Sequence | GATTGCTGCACCCCACTTTG | AGATCCACCACCAGCCAAAG | Start: 235 |
| Tm | 60.038 | 59.961 | End: 391 |
| Size | 20 | 20 | 157 |
| Primer 3 details |
|---|
| Sequence | TTGCTGCACCCCACTTTGTA | AGATCCACCACCAGCCAAAG | Start: 237 |
| Tm | 60.106 | 59.961 | End: 391 |
| Size | 20 | 20 | 155 |
| Primer 4 details |
|---|
| Sequence | GATTGCTGCACCCCACTTTG | AAATGACGGGACCGGTGATC | Start: 235 |
| Tm | 60.038 | 60.108 | End: 491 |
| Size | 20 | 20 | 257 |
| Primer 5 details |
|---|
| Sequence | GATTGCTGCACCCCACTTTG | GAAATGACGGGACCGGTGAT | Start: 235 |
| Tm | 60.038 | 60.108 | End: 492 |
| Size | 20 | 20 | 258 |
(AT)9: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGGGGAATTATAGCGCCG | ACGTCCCTCATCCTCACTCA | Start: 3095 |
| Tm | 59.965 | 59.96 | End: 3345 |
| Size | 20 | 20 | 251 |
| Primer 2 details |
|---|
| Sequence | GATTGCTGCACCCCACTTTG | AGATCCACCACCAGCCAAAG | Start: 235 |
| Tm | 60.038 | 59.961 | End: 391 |
| Size | 20 | 20 | 157 |
| Primer 3 details |
|---|
| Sequence | TTGCTGCACCCCACTTTGTA | AGATCCACCACCAGCCAAAG | Start: 237 |
| Tm | 60.106 | 59.961 | End: 391 |
| Size | 20 | 20 | 155 |
| Primer 4 details |
|---|
| Sequence | GATTGCTGCACCCCACTTTG | AAATGACGGGACCGGTGATC | Start: 235 |
| Tm | 60.038 | 60.108 | End: 491 |
| Size | 20 | 20 | 257 |
| Primer 5 details |
|---|
| Sequence | GATTGCTGCACCCCACTTTG | GAAATGACGGGACCGGTGAT | Start: 235 |
| Tm | 60.038 | 60.108 | End: 492 |
| Size | 20 | 20 | 258 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGGGGAATTATAGCGCCG | ACGTCCCTCATCCTCACTCA | Start: 3095 |
| Tm | 59.965 | 59.96 | End: 3345 |
| Size | 20 | 20 | 251 |
| Primer 2 details |
|---|
| Sequence | GATTGCTGCACCCCACTTTG | AGATCCACCACCAGCCAAAG | Start: 235 |
| Tm | 60.038 | 59.961 | End: 391 |
| Size | 20 | 20 | 157 |
| Primer 3 details |
|---|
| Sequence | TTGCTGCACCCCACTTTGTA | AGATCCACCACCAGCCAAAG | Start: 237 |
| Tm | 60.106 | 59.961 | End: 391 |
| Size | 20 | 20 | 155 |
| Primer 4 details |
|---|
| Sequence | GATTGCTGCACCCCACTTTG | AAATGACGGGACCGGTGATC | Start: 235 |
| Tm | 60.038 | 60.108 | End: 491 |
| Size | 20 | 20 | 257 |
| Primer 5 details |
|---|
| Sequence | GATTGCTGCACCCCACTTTG | GAAATGACGGGACCGGTGAT | Start: 235 |
| Tm | 60.038 | 60.108 | End: 492 |
| Size | 20 | 20 | 258 |
(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGGGGAATTATAGCGCCG | ACGTCCCTCATCCTCACTCA | Start: 3095 |
| Tm | 59.965 | 59.96 | End: 3345 |
| Size | 20 | 20 | 251 |
| Primer 2 details |
|---|
| Sequence | GATTGCTGCACCCCACTTTG | AGATCCACCACCAGCCAAAG | Start: 235 |
| Tm | 60.038 | 59.961 | End: 391 |
| Size | 20 | 20 | 157 |
| Primer 3 details |
|---|
| Sequence | TTGCTGCACCCCACTTTGTA | AGATCCACCACCAGCCAAAG | Start: 237 |
| Tm | 60.106 | 59.961 | End: 391 |
| Size | 20 | 20 | 155 |
| Primer 4 details |
|---|
| Sequence | GATTGCTGCACCCCACTTTG | AAATGACGGGACCGGTGATC | Start: 235 |
| Tm | 60.038 | 60.108 | End: 491 |
| Size | 20 | 20 | 257 |
| Primer 5 details |
|---|
| Sequence | GATTGCTGCACCCCACTTTG | GAAATGACGGGACCGGTGAT | Start: 235 |
| Tm | 60.038 | 60.108 | End: 492 |
| Size | 20 | 20 | 258 |
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