ssr_primer
Primer Details for TRINITY_DN12328_c1_g1_i2 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAGGCAAGACAGGTGGCAAG | GATAGTGGCCCTTGTCCGAC | Start: 326 |
| Tm | 59.963 | 60.179 | End: 562 |
| Size | 20 | 20 | 237 |
| Primer 2 details |
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| Sequence | TAGGCAAGACAGGTGGCAAG | CGGTCCCTACCTTTAAGGCC | Start: 326 |
| Tm | 59.963 | 59.82 | End: 591 |
| Size | 20 | 20 | 266 |
| Primer 3 details |
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| Sequence | TCCTTGCGTGGGAAGGAAAG | GCGGAAGTGTGCATTGGTTT | Start: 1218 |
| Tm | 60.251 | 59.968 | End: 1493 |
| Size | 20 | 20 | 276 |
| Primer 4 details |
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| Sequence | CTCCTTGCGTGGGAAGGAAA | GCGGAAGTGTGCATTGGTTT | Start: 1217 |
| Tm | 60.251 | 59.968 | End: 1493 |
| Size | 20 | 20 | 277 |
| Primer 5 details |
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| Sequence | AGTGCCAACCACACTGCTAG | GGATTGCAGCCTCTTCCCTT | Start: 999 |
| Tm | 60.25 | 60.034 | End: 1217 |
| Size | 20 | 20 | 219 |
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