ssr_primer
Primer Details for TRINITY_DN12298_c0_g1_i1 |
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| (TTC)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCTTCCATCGCCCAAAGGT | TCCTGATTCGCCCTACCTGA | Start: 530 |
| Tm | 60.035 | 60.033 | End: 728 |
| Size | 20 | 20 | 199 |
| Primer 2 details |
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| Sequence | CGGGGAATTGTTTGGCGTTT | ACCTTTGGGCGATGGAAGAG | Start: 274 |
| Tm | 59.967 | 60.035 | End: 549 |
| Size | 20 | 20 | 276 |
| Primer 3 details |
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| Sequence | CGGGGAATTGTTTGGCGTTT | GGGAGTGGTAAGGGAACAGC | Start: 274 |
| Tm | 59.967 | 60.035 | End: 455 |
| Size | 20 | 20 | 182 |
| Primer 4 details |
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| Sequence | GTGCCTCTAGGTATTCCCGC | ACCTTTGGGCGATGGAAGAG | Start: 296 |
| Tm | 59.966 | 60.035 | End: 549 |
| Size | 20 | 20 | 254 |
| Primer 5 details |
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| Sequence | GTGCCTCTAGGTATTCCCGC | GGGAGTGGTAAGGGAACAGC | Start: 296 |
| Tm | 59.966 | 60.035 | End: 455 |
| Size | 20 | 20 | 160 |
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