ssr_primer
Primer Details for TRINITY_DN12260_c1_g1_i5 |
|---|
| (GAA)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTCCCTCTAATGGGGGTG | TCGTCCTGCAACCTTCAGTC | Start: 786 |
| Tm | 60.03 | 59.966 | End: 1035 |
| Size | 20 | 20 | 250 |
| Primer 2 details |
|---|
| Sequence | GCGCTGTTTGGGTTTGGAAT | TGCAATGCTTGTGGTGTTCG | Start: 481 |
| Tm | 59.967 | 59.969 | End: 677 |
| Size | 20 | 20 | 197 |
| Primer 3 details |
|---|
| Sequence | TCCTCCCTCTAATGGGGGTG | CTCGTCCTGCAACCTTCAGT | Start: 786 |
| Tm | 60.03 | 59.966 | End: 1036 |
| Size | 20 | 20 | 251 |
| Primer 4 details |
|---|
| Sequence | TGGGAATTCGAGTGCAACGA | TGCAATGCTTGTGGTGTTCG | Start: 577 |
| Tm | 59.966 | 59.969 | End: 677 |
| Size | 20 | 20 | 101 |
| Primer 5 details |
|---|
| Sequence | AGCTCTGGTTGCACGCTTAT | TGCAATGCTTGTGGTGTTCG | Start: 502 |
| Tm | 60.036 | 59.969 | End: 677 |
| Size | 20 | 20 | 176 |
(A)12cctaaaacctttcccaagttgcccccaccttcccctgtacaagaaagacaaccacctaaaaactatttcatactaaacaca(AACC)5acacacacatcacccatctttcttctc(CTT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTCCCTCTAATGGGGGTG | TCGTCCTGCAACCTTCAGTC | Start: 786 |
| Tm | 60.03 | 59.966 | End: 1035 |
| Size | 20 | 20 | 250 |
| Primer 2 details |
|---|
| Sequence | GCGCTGTTTGGGTTTGGAAT | TGCAATGCTTGTGGTGTTCG | Start: 481 |
| Tm | 59.967 | 59.969 | End: 677 |
| Size | 20 | 20 | 197 |
| Primer 3 details |
|---|
| Sequence | TCCTCCCTCTAATGGGGGTG | CTCGTCCTGCAACCTTCAGT | Start: 786 |
| Tm | 60.03 | 59.966 | End: 1036 |
| Size | 20 | 20 | 251 |
| Primer 4 details |
|---|
| Sequence | TGGGAATTCGAGTGCAACGA | TGCAATGCTTGTGGTGTTCG | Start: 577 |
| Tm | 59.966 | 59.969 | End: 677 |
| Size | 20 | 20 | 101 |
| Primer 5 details |
|---|
| Sequence | AGCTCTGGTTGCACGCTTAT | TGCAATGCTTGTGGTGTTCG | Start: 502 |
| Tm | 60.036 | 59.969 | End: 677 |
| Size | 20 | 20 | 176 |
(T)11caaaag(GAA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTCCCTCTAATGGGGGTG | TCGTCCTGCAACCTTCAGTC | Start: 786 |
| Tm | 60.03 | 59.966 | End: 1035 |
| Size | 20 | 20 | 250 |
| Primer 2 details |
|---|
| Sequence | GCGCTGTTTGGGTTTGGAAT | TGCAATGCTTGTGGTGTTCG | Start: 481 |
| Tm | 59.967 | 59.969 | End: 677 |
| Size | 20 | 20 | 197 |
| Primer 3 details |
|---|
| Sequence | TCCTCCCTCTAATGGGGGTG | CTCGTCCTGCAACCTTCAGT | Start: 786 |
| Tm | 60.03 | 59.966 | End: 1036 |
| Size | 20 | 20 | 251 |
| Primer 4 details |
|---|
| Sequence | TGGGAATTCGAGTGCAACGA | TGCAATGCTTGTGGTGTTCG | Start: 577 |
| Tm | 59.966 | 59.969 | End: 677 |
| Size | 20 | 20 | 101 |
| Primer 5 details |
|---|
| Sequence | AGCTCTGGTTGCACGCTTAT | TGCAATGCTTGTGGTGTTCG | Start: 502 |
| Tm | 60.036 | 59.969 | End: 677 |
| Size | 20 | 20 | 176 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTCCCTCTAATGGGGGTG | TCGTCCTGCAACCTTCAGTC | Start: 786 |
| Tm | 60.03 | 59.966 | End: 1035 |
| Size | 20 | 20 | 250 |
| Primer 2 details |
|---|
| Sequence | GCGCTGTTTGGGTTTGGAAT | TGCAATGCTTGTGGTGTTCG | Start: 481 |
| Tm | 59.967 | 59.969 | End: 677 |
| Size | 20 | 20 | 197 |
| Primer 3 details |
|---|
| Sequence | TCCTCCCTCTAATGGGGGTG | CTCGTCCTGCAACCTTCAGT | Start: 786 |
| Tm | 60.03 | 59.966 | End: 1036 |
| Size | 20 | 20 | 251 |
| Primer 4 details |
|---|
| Sequence | TGGGAATTCGAGTGCAACGA | TGCAATGCTTGTGGTGTTCG | Start: 577 |
| Tm | 59.966 | 59.969 | End: 677 |
| Size | 20 | 20 | 101 |
| Primer 5 details |
|---|
| Sequence | AGCTCTGGTTGCACGCTTAT | TGCAATGCTTGTGGTGTTCG | Start: 502 |
| Tm | 60.036 | 59.969 | End: 677 |
| Size | 20 | 20 | 176 |
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