ssr_primer
Primer Details for TRINITY_DN12209_c2_g2_i2 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCCCCGCACTTTCTCACTT | TAAGGGCCGAGCACAATGTT | Start: 167 |
| Tm | 59.965 | 59.962 | End: 349 |
| Size | 20 | 20 | 183 |
| Primer 2 details |
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| Sequence | ACCTTTCCTCTCGCCTCTCT | TAAGGGCCGAGCACAATGTT | Start: 120 |
| Tm | 59.96 | 59.962 | End: 349 |
| Size | 20 | 20 | 230 |
| Primer 3 details |
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| Sequence | ATCTAGGCCGCTGTGGTTTC | AAGTGAGAAAGTGCGGGGAG | Start: 20 |
| Tm | 60.108 | 59.965 | End: 186 |
| Size | 20 | 20 | 167 |
| Primer 4 details |
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| Sequence | ATCTAGGCCGCTGTGGTTTC | AGAGAGGCGAGAGGAAAGGT | Start: 20 |
| Tm | 60.108 | 59.96 | End: 139 |
| Size | 20 | 20 | 120 |
| Primer 5 details |
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| Sequence | AAAGCTCCCAATCTAGGCCG | AAGTGAGAAAGTGCGGGGAG | Start: 10 |
| Tm | 59.819 | 59.965 | End: 186 |
| Size | 20 | 20 | 177 |
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