ssr_primer
Primer Details for TRINITY_DN12177_c0_g2_i2 |
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| (GAC)7acccttccactttcaccaccaccgaccccttttctctct(TTC)8: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTCTCCTGCACAATCCGCA | TCGGGCTGGGAGTATTGAGA | Start: 292 |
| Tm | 60.035 | 60.033 | End: 546 |
| Size | 20 | 20 | 255 |
| Primer 2 details |
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| Sequence | TCTACCCTTCCCGACGACAT | TGCGGATTGTGCAGGAGAAT | Start: 200 |
| Tm | 60.034 | 60.035 | End: 311 |
| Size | 20 | 20 | 112 |
| Primer 3 details |
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| Sequence | CCACCGACCCCTTTTCTCTC | ATGTCGTCGGGAAGGGTAGA | Start: 103 |
| Tm | 60.036 | 60.034 | End: 219 |
| Size | 20 | 20 | 117 |
| Primer 4 details |
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| Sequence | CCACCGACCCCTTTTCTCTC | TGCGGATTGTGCAGGAGAAT | Start: 103 |
| Tm | 60.036 | 60.035 | End: 311 |
| Size | 20 | 20 | 209 |
| Primer 5 details |
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| Sequence | GCTCGCTGTTTGTTGTCCTG | TCGGGCTGGGAGTATTGAGA | Start: 415 |
| Tm | 60.041 | 60.033 | End: 546 |
| Size | 20 | 20 | 132 |
(TCTGCT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTCTCCTGCACAATCCGCA | TCGGGCTGGGAGTATTGAGA | Start: 292 |
| Tm | 60.035 | 60.033 | End: 546 |
| Size | 20 | 20 | 255 |
| Primer 2 details |
|---|
| Sequence | TCTACCCTTCCCGACGACAT | TGCGGATTGTGCAGGAGAAT | Start: 200 |
| Tm | 60.034 | 60.035 | End: 311 |
| Size | 20 | 20 | 112 |
| Primer 3 details |
|---|
| Sequence | CCACCGACCCCTTTTCTCTC | ATGTCGTCGGGAAGGGTAGA | Start: 103 |
| Tm | 60.036 | 60.034 | End: 219 |
| Size | 20 | 20 | 117 |
| Primer 4 details |
|---|
| Sequence | CCACCGACCCCTTTTCTCTC | TGCGGATTGTGCAGGAGAAT | Start: 103 |
| Tm | 60.036 | 60.035 | End: 311 |
| Size | 20 | 20 | 209 |
| Primer 5 details |
|---|
| Sequence | GCTCGCTGTTTGTTGTCCTG | TCGGGCTGGGAGTATTGAGA | Start: 415 |
| Tm | 60.041 | 60.033 | End: 546 |
| Size | 20 | 20 | 132 |
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