ssr_primer
Primer Details for TRINITY_DN12163_c0_g3_i5 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCGATGTCCCAAAAGAGCT | AGGAGCTGAACATCTTGGCC | Start: 519 |
| Tm | 59.963 | 60.035 | End: 691 |
| Size | 20 | 20 | 173 |
| Primer 2 details |
|---|
| Sequence | TGCGATGTCCCAAAAGAGCT | TCTTCAGGTCGACCTTTCGC | Start: 519 |
| Tm | 59.963 | 60.039 | End: 637 |
| Size | 20 | 20 | 119 |
| Primer 3 details |
|---|
| Sequence | CGGCGGTTCAGCTATGTTTG | AGGAGCTGAACATCTTGGCC | Start: 567 |
| Tm | 59.903 | 60.035 | End: 691 |
| Size | 20 | 20 | 125 |
| Primer 4 details |
|---|
| Sequence | CCGTCGATCTCACTCCACAG | AGCTCTTTTGGGACATCGCA | Start: 386 |
| Tm | 59.9 | 59.963 | End: 538 |
| Size | 20 | 20 | 153 |
| Primer 5 details |
|---|
| Sequence | CCGTCGATCTCACTCCACAG | TCTTCAGGTCGACCTTTCGC | Start: 386 |
| Tm | 59.9 | 60.039 | End: 637 |
| Size | 20 | 20 | 252 |
(A)11taaactctctctcctagtactctgtctggctgtttgtttgttttgcttttaaaatgtctgtttaattattacccaaaaaag(A)12ctataatgggctttgcttttt(A)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCGATGTCCCAAAAGAGCT | AGGAGCTGAACATCTTGGCC | Start: 519 |
| Tm | 59.963 | 60.035 | End: 691 |
| Size | 20 | 20 | 173 |
| Primer 2 details |
|---|
| Sequence | TGCGATGTCCCAAAAGAGCT | TCTTCAGGTCGACCTTTCGC | Start: 519 |
| Tm | 59.963 | 60.039 | End: 637 |
| Size | 20 | 20 | 119 |
| Primer 3 details |
|---|
| Sequence | CGGCGGTTCAGCTATGTTTG | AGGAGCTGAACATCTTGGCC | Start: 567 |
| Tm | 59.903 | 60.035 | End: 691 |
| Size | 20 | 20 | 125 |
| Primer 4 details |
|---|
| Sequence | CCGTCGATCTCACTCCACAG | AGCTCTTTTGGGACATCGCA | Start: 386 |
| Tm | 59.9 | 59.963 | End: 538 |
| Size | 20 | 20 | 153 |
| Primer 5 details |
|---|
| Sequence | CCGTCGATCTCACTCCACAG | TCTTCAGGTCGACCTTTCGC | Start: 386 |
| Tm | 59.9 | 60.039 | End: 637 |
| Size | 20 | 20 | 252 |
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