ssr_primer
Primer Details for TRINITY_DN12131_c1_g3_i3 |
|---|
| (TA)7catacatat(AC)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGCTTGGCTACTTCGCTT | TGTGTAGCCCAGCAATGAGG | Start: 763 |
| Tm | 60.035 | 60.035 | End: 1038 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
|---|
| Sequence | GTACCACTAGACGGGTCCCT | TGTCAAATTGCCGAGCTCCT | Start: 738 |
| Tm | 60.034 | 59.963 | End: 884 |
| Size | 20 | 20 | 147 |
| Primer 3 details |
|---|
| Sequence | ATGGCTTGGCTACTTCGCTT | TGTCAAATTGCCGAGCTCCT | Start: 763 |
| Tm | 60.035 | 59.963 | End: 884 |
| Size | 20 | 20 | 122 |
| Primer 4 details |
|---|
| Sequence | AGGAGCTCGGCAATTTGACA | TGTGTAGCCCAGCAATGAGG | Start: 865 |
| Tm | 59.963 | 60.035 | End: 1038 |
| Size | 20 | 20 | 174 |
| Primer 5 details |
|---|
| Sequence | GTACCACTAGACGGGTCCCT | GCAGCATCACTCGACCCATA | Start: 738 |
| Tm | 60.034 | 59.895 | End: 981 |
| Size | 20 | 20 | 244 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGCTTGGCTACTTCGCTT | TGTGTAGCCCAGCAATGAGG | Start: 763 |
| Tm | 60.035 | 60.035 | End: 1038 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
|---|
| Sequence | GTACCACTAGACGGGTCCCT | TGTCAAATTGCCGAGCTCCT | Start: 738 |
| Tm | 60.034 | 59.963 | End: 884 |
| Size | 20 | 20 | 147 |
| Primer 3 details |
|---|
| Sequence | ATGGCTTGGCTACTTCGCTT | TGTCAAATTGCCGAGCTCCT | Start: 763 |
| Tm | 60.035 | 59.963 | End: 884 |
| Size | 20 | 20 | 122 |
| Primer 4 details |
|---|
| Sequence | AGGAGCTCGGCAATTTGACA | TGTGTAGCCCAGCAATGAGG | Start: 865 |
| Tm | 59.963 | 60.035 | End: 1038 |
| Size | 20 | 20 | 174 |
| Primer 5 details |
|---|
| Sequence | GTACCACTAGACGGGTCCCT | GCAGCATCACTCGACCCATA | Start: 738 |
| Tm | 60.034 | 59.895 | End: 981 |
| Size | 20 | 20 | 244 |
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