ssr_primer
Primer Details for TRINITY_DN12066_c0_g3_i2 |
|---|
| (TAA)5tacttatcaattccctcacccaaccattttgacttacgtctctccatttcccacaccaaccatcttttttctaccctcaactctatt(TCC)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTGTTGAGGCAGAGGAGCT | CATCTCCCACCTTTCCCCAC | Start: 3731 |
| Tm | 59.964 | 60.034 | End: 4002 |
| Size | 20 | 20 | 272 |
| Primer 2 details |
|---|
| Sequence | GTGGGGAAAGGTGGGAGATG | TCCCCAAATCCAACGTTCGT | Start: 3983 |
| Tm | 60.034 | 59.891 | End: 4128 |
| Size | 20 | 20 | 146 |
| Primer 3 details |
|---|
| Sequence | TTTTCGGAGCTGCACTTCCT | AGCTCCTCTGCCTCAACAAC | Start: 3481 |
| Tm | 59.891 | 59.964 | End: 3750 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
|---|
| Sequence | GTGGGGAAAGGTGGGAGATG | TGGCCGAAATTCAGAACCCA | Start: 3983 |
| Tm | 60.034 | 59.889 | End: 4214 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | AAGTCACCACTAGCATCGCC | TGAAAATGGCGGACATCCCA | Start: 3022 |
| Tm | 60.108 | 59.961 | End: 3275 |
| Size | 20 | 20 | 254 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTGTTGAGGCAGAGGAGCT | CATCTCCCACCTTTCCCCAC | Start: 3731 |
| Tm | 59.964 | 60.034 | End: 4002 |
| Size | 20 | 20 | 272 |
| Primer 2 details |
|---|
| Sequence | GTGGGGAAAGGTGGGAGATG | TCCCCAAATCCAACGTTCGT | Start: 3983 |
| Tm | 60.034 | 59.891 | End: 4128 |
| Size | 20 | 20 | 146 |
| Primer 3 details |
|---|
| Sequence | TTTTCGGAGCTGCACTTCCT | AGCTCCTCTGCCTCAACAAC | Start: 3481 |
| Tm | 59.891 | 59.964 | End: 3750 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
|---|
| Sequence | GTGGGGAAAGGTGGGAGATG | TGGCCGAAATTCAGAACCCA | Start: 3983 |
| Tm | 60.034 | 59.889 | End: 4214 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | AAGTCACCACTAGCATCGCC | TGAAAATGGCGGACATCCCA | Start: 3022 |
| Tm | 60.108 | 59.961 | End: 3275 |
| Size | 20 | 20 | 254 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTGTTGAGGCAGAGGAGCT | CATCTCCCACCTTTCCCCAC | Start: 3731 |
| Tm | 59.964 | 60.034 | End: 4002 |
| Size | 20 | 20 | 272 |
| Primer 2 details |
|---|
| Sequence | GTGGGGAAAGGTGGGAGATG | TCCCCAAATCCAACGTTCGT | Start: 3983 |
| Tm | 60.034 | 59.891 | End: 4128 |
| Size | 20 | 20 | 146 |
| Primer 3 details |
|---|
| Sequence | TTTTCGGAGCTGCACTTCCT | AGCTCCTCTGCCTCAACAAC | Start: 3481 |
| Tm | 59.891 | 59.964 | End: 3750 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
|---|
| Sequence | GTGGGGAAAGGTGGGAGATG | TGGCCGAAATTCAGAACCCA | Start: 3983 |
| Tm | 60.034 | 59.889 | End: 4214 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | AAGTCACCACTAGCATCGCC | TGAAAATGGCGGACATCCCA | Start: 3022 |
| Tm | 60.108 | 59.961 | End: 3275 |
| Size | 20 | 20 | 254 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTGTTGAGGCAGAGGAGCT | CATCTCCCACCTTTCCCCAC | Start: 3731 |
| Tm | 59.964 | 60.034 | End: 4002 |
| Size | 20 | 20 | 272 |
| Primer 2 details |
|---|
| Sequence | GTGGGGAAAGGTGGGAGATG | TCCCCAAATCCAACGTTCGT | Start: 3983 |
| Tm | 60.034 | 59.891 | End: 4128 |
| Size | 20 | 20 | 146 |
| Primer 3 details |
|---|
| Sequence | TTTTCGGAGCTGCACTTCCT | AGCTCCTCTGCCTCAACAAC | Start: 3481 |
| Tm | 59.891 | 59.964 | End: 3750 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
|---|
| Sequence | GTGGGGAAAGGTGGGAGATG | TGGCCGAAATTCAGAACCCA | Start: 3983 |
| Tm | 60.034 | 59.889 | End: 4214 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | AAGTCACCACTAGCATCGCC | TGAAAATGGCGGACATCCCA | Start: 3022 |
| Tm | 60.108 | 59.961 | End: 3275 |
| Size | 20 | 20 | 254 |
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