ssr_primer
Primer Details for TRINITY_DN12064_c1_g1_i12 |
|---|
| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACAACCGGTGAGGAAGAGG | AATTCTCCACCGAACGCCTT | Start: 2688 |
| Tm | 60.037 | 59.964 | End: 2884 |
| Size | 20 | 20 | 197 |
| Primer 2 details |
|---|
| Sequence | AACGCACTGCTCTCTTCCTC | CCTCTTCCTCACCGGTTGTC | Start: 2476 |
| Tm | 60.038 | 60.037 | End: 2707 |
| Size | 20 | 20 | 232 |
| Primer 3 details |
|---|
| Sequence | GGGGTAGACTTGTGGTTGGG | CACATGGTTGCTCACAGTGC | Start: 625 |
| Tm | 59.962 | 60.04 | End: 786 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
|---|
| Sequence | ATGAGACATTGAAGCGGCCA | GCACTCTTTTGGCAGCAACA | Start: 1036 |
| Tm | 60.035 | 59.897 | End: 1173 |
| Size | 20 | 20 | 138 |
| Primer 5 details |
|---|
| Sequence | GGAAGAGGAGGAGGAGGAGG | AATTCTCCACCGAACGCCTT | Start: 2700 |
| Tm | 60.106 | 59.964 | End: 2884 |
| Size | 20 | 20 | 185 |
(GAG)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACAACCGGTGAGGAAGAGG | AATTCTCCACCGAACGCCTT | Start: 2688 |
| Tm | 60.037 | 59.964 | End: 2884 |
| Size | 20 | 20 | 197 |
| Primer 2 details |
|---|
| Sequence | AACGCACTGCTCTCTTCCTC | CCTCTTCCTCACCGGTTGTC | Start: 2476 |
| Tm | 60.038 | 60.037 | End: 2707 |
| Size | 20 | 20 | 232 |
| Primer 3 details |
|---|
| Sequence | GGGGTAGACTTGTGGTTGGG | CACATGGTTGCTCACAGTGC | Start: 625 |
| Tm | 59.962 | 60.04 | End: 786 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
|---|
| Sequence | ATGAGACATTGAAGCGGCCA | GCACTCTTTTGGCAGCAACA | Start: 1036 |
| Tm | 60.035 | 59.897 | End: 1173 |
| Size | 20 | 20 | 138 |
| Primer 5 details |
|---|
| Sequence | GGAAGAGGAGGAGGAGGAGG | AATTCTCCACCGAACGCCTT | Start: 2700 |
| Tm | 60.106 | 59.964 | End: 2884 |
| Size | 20 | 20 | 185 |
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