ssr_primer
Primer Details for TRINITY_DN11935_c3_g3_i2 |
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| (GCA)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCCAAGGCGTCTACATCTT | ATGCCGACGGAGAAGATGTC | Start: 137 |
| Tm | 60.108 | 59.897 | End: 252 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
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| Sequence | GTTTGATGCCTTGCTGGACG | GAACCATCTGCTGCTGTTGC | Start: 671 |
| Tm | 60.11 | 60.109 | End: 906 |
| Size | 20 | 20 | 236 |
| Primer 3 details |
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| Sequence | GTTTGATGCCTTGCTGGACG | TGGAACCATCTGCTGCTGTT | Start: 671 |
| Tm | 60.11 | 59.889 | End: 908 |
| Size | 20 | 20 | 238 |
| Primer 4 details |
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| Sequence | GTTTGATGCCTTGCTGGACG | AACAATGGTTGACCCTCCCC | Start: 671 |
| Tm | 60.11 | 59.886 | End: 874 |
| Size | 20 | 20 | 204 |
| Primer 5 details |
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| Sequence | GCCCATTAGCATCCAGGTCA | ATGCCGACGGAGAAGATGTC | Start: 82 |
| Tm | 59.817 | 59.897 | End: 252 |
| Size | 20 | 20 | 171 |
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