ssr_primer
Primer Details for TRINITY_DN11630_c2_g5_i2 |
|---|
| (T)11aagaaaaaacagcagctgtgctaaaaag(A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGAAGAAGAAGGGTGGCCG | TTCGGCAAGGTAAGGTCCAC | Start: 433 |
| Tm | 59.963 | 59.964 | End: 640 |
| Size | 20 | 20 | 208 |
| Primer 2 details |
|---|
| Sequence | GTGGACCTTACCTTGCCGAA | CCTTCTCCCGAGCACGAATT | Start: 621 |
| Tm | 59.964 | 60.108 | End: 753 |
| Size | 20 | 20 | 133 |
| Primer 3 details |
|---|
| Sequence | AAGAAGAAGAAGGGTGGCCG | AAACTCTATCGTCGGTGCCG | Start: 433 |
| Tm | 59.963 | 60.179 | End: 676 |
| Size | 20 | 20 | 244 |
| Primer 4 details |
|---|
| Sequence | AAGAAGAAGAAGGGTGGCCG | CTTCGGCAAGGTAAGGTCCA | Start: 433 |
| Tm | 59.963 | 59.676 | End: 641 |
| Size | 20 | 20 | 209 |
| Primer 5 details |
|---|
| Sequence | TGGACCTTACCTTGCCGAAG | CCTTCTCCCGAGCACGAATT | Start: 622 |
| Tm | 59.676 | 60.108 | End: 753 |
| Size | 20 | 20 | 132 |
(GAA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGAAGAAGAAGGGTGGCCG | TTCGGCAAGGTAAGGTCCAC | Start: 433 |
| Tm | 59.963 | 59.964 | End: 640 |
| Size | 20 | 20 | 208 |
| Primer 2 details |
|---|
| Sequence | GTGGACCTTACCTTGCCGAA | CCTTCTCCCGAGCACGAATT | Start: 621 |
| Tm | 59.964 | 60.108 | End: 753 |
| Size | 20 | 20 | 133 |
| Primer 3 details |
|---|
| Sequence | AAGAAGAAGAAGGGTGGCCG | AAACTCTATCGTCGGTGCCG | Start: 433 |
| Tm | 59.963 | 60.179 | End: 676 |
| Size | 20 | 20 | 244 |
| Primer 4 details |
|---|
| Sequence | AAGAAGAAGAAGGGTGGCCG | CTTCGGCAAGGTAAGGTCCA | Start: 433 |
| Tm | 59.963 | 59.676 | End: 641 |
| Size | 20 | 20 | 209 |
| Primer 5 details |
|---|
| Sequence | TGGACCTTACCTTGCCGAAG | CCTTCTCCCGAGCACGAATT | Start: 622 |
| Tm | 59.676 | 60.108 | End: 753 |
| Size | 20 | 20 | 132 |
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