ssr_primer
Primer Details for TRINITY_DN11576_c5_g1_i1 |
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| (A)19: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGTTGTAACAGCGGAAGA | CCACCTGGTCGTAAACGTCA | Start: 303 |
| Tm | 59.964 | 59.968 | End: 420 |
| Size | 20 | 20 | 118 |
| Primer 2 details |
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| Sequence | GGACAGTCGACATTGGGGTT | CCACCTGGTCGTAAACGTCA | Start: 289 |
| Tm | 59.964 | 59.968 | End: 420 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
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| Sequence | CGGTGCCATGACTCCTTTCT | TCTTCCGCTGTTACAACCCC | Start: 59 |
| Tm | 60.036 | 59.964 | End: 322 |
| Size | 20 | 20 | 264 |
| Primer 4 details |
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| Sequence | TCGGTGCCATGACTCCTTTC | TCTTCCGCTGTTACAACCCC | Start: 58 |
| Tm | 60.036 | 59.964 | End: 322 |
| Size | 20 | 20 | 265 |
| Primer 5 details |
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| Sequence | CGGTGCCATGACTCCTTTCT | AACCCCAATGTCGACTGTCC | Start: 59 |
| Tm | 60.036 | 59.964 | End: 308 |
| Size | 20 | 20 | 250 |
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