Primer Details for TRINITY_DN11371_c0_g2_i1 |
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| (TA)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACTAGTTAGGGTGTCATTGTAAGT | TTCGGCTTTGGACCACCATT | Start: 139 |
| Tm | 59.731 | 60.179 | End: 338 |
| Size | 26 | 20 | 200 |
| Primer 2 details |
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| Sequence | ACACTAGTTAGGGTGTCATTGTAAGT | TAAGGTGGGTTCGGCTTTGG | Start: 139 |
| Tm | 59.731 | 60.251 | End: 347 |
| Size | 26 | 20 | 209 |
| Primer 3 details |
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| Sequence | ACACTAGTTAGGGTGTCATTGTAAGT | GACTAAGGTGGGTTCGGCTT | Start: 139 |
| Tm | 59.731 | 59.676 | End: 350 |
| Size | 26 | 20 | 212 |
| Primer 4 details |
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| Sequence | ACACTAGTTAGGGTGTCATTGTAA | TTCGGCTTTGGACCACCATT | Start: 139 |
| Tm | 57.325 | 60.179 | End: 338 |
| Size | 24 | 20 | 200 |
| Primer 5 details |
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| Sequence | CACTAGTTAGGGTGTCATTGTAAGT | TTCGGCTTTGGACCACCATT | Start: 140 |
| Tm | 58.302 | 60.179 | End: 338 |
| Size | 25 | 20 | 199 |
