ssr_primer
Primer Details for TRINITY_DN11069_c6_g1_i2 |
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| (AT)6tatgatatttgttcatcttc(TCT)5tcgtctttctttctttccttc(TTCT)5(TTC)6*ctcttcca(CT)6tcttcacaggtcactggt(GA)7: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCAGCTACTCCCTTCCCC | AAGTGAGCGGAATGAGGGTG | Start: 395 |
| Tm | 60.032 | 60.036 | End: 578 |
| Size | 20 | 20 | 184 |
| Primer 2 details |
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| Sequence | GGAAGTTGGGCCCTCGTAAA | TGTCGATCAATTGCAGGCCT | Start: 3022 |
| Tm | 59.963 | 60.035 | End: 3187 |
| Size | 20 | 20 | 166 |
| Primer 3 details |
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| Sequence | CTTCCTGTTTCTGGCCCCAT | AAGTGAGCGGAATGAGGGTG | Start: 435 |
| Tm | 59.96 | 60.036 | End: 578 |
| Size | 20 | 20 | 144 |
| Primer 4 details |
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| Sequence | TGTTGGTTTCATGCGCAAGG | AGGTGCTTGTAAAGGTGCGA | Start: 4301 |
| Tm | 59.968 | 59.892 | End: 4420 |
| Size | 20 | 20 | 120 |
| Primer 5 details |
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| Sequence | ATCATTCCCTCCCCCTCCTC | AAGTGAGCGGAATGAGGGTG | Start: 331 |
| Tm | 60.104 | 60.036 | End: 578 |
| Size | 20 | 20 | 248 |
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